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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 21 Peptide

Catalog No. PV4535
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800 x 20μL, 800 x 20 μL Assays
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PV4535 800 x 20μL, 800 x 20 μL Assays
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Catalog No. PV4535 Supplier Thermo Scientific™ Supplier No. PV4535
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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This substrate is based upon CDC25C, containing Ser-216.

Specifications

Description Z'-LYTE™ Ser/Thr 21 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Serine/Threonine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Serine/Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis
Product Line Z ́-LYTE
For Use With (Application) Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acutelyte™ kinase assays, enzyme and protein activity assays, protein assays and analysis, kinase activity, FRET
Includes Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer
Sample Volume 800 x 20μL
Assay Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Product Type Z'-LYTE™ Ser/Thr 21 Peptide Kinase Assay Kit
Quantity 800 x 20μL, 800 x 20 μL Assays
Format 384-well plate
Excitation/Emission 405/465, 535 nm
Label or Dye Methylcoumarin
No. of Reactions 800 Reactions
Readout End Point
Shipping Condition Dry Ice
Source Based on CDC25C Residues 207-225, Centered at Ser-216 (SRSGLYRSPSMPENLNRPRL)
Substrate Properties Peptide-Based Substrate
Target Entry MARK1, MARK2, BRSK1
Target Enzyme Serine/Threonine Kinases
Validated Kinases BRSK1, MARK1, MARK2
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For Research Use Only. Not for use in diagnostic procedures.