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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 15 Peptide

Catalog No. PV3799
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800 x 20μL, 800 x 20 μL Assays
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PV3799 800 x 20μL, 800 x 20 μL Assays
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Catalog No. PV3799 Supplier Thermo Scientific™ Supplier No. PV3799
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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon EGFR, containing Thr-693.

Specifications

Description Z'-LYTE™ Kinase Assay Kit - Ser/Thr 15 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout,
Product Line Z ́-LYTE
For Use With (Application) Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation, FRET
Includes All necessary reagents for performing primary or secondary screens
Sample Volume 800 x 20μL
Assay Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Product Type Z'-LYTE™ Kinase Assay Kit - Ser/Thr 15 Peptide
Quantity 800 x 20μL, 800 x 20 μL Assays
Format 384-well plate
Excitation/Emission 405/465, 535 nm
Label or Dye Methylcoumarin
No. of Reactions 800 Reactions
Readout End Point
References J. Cell. Phys.(1995), v. 162, p434
Research Category Signal Transduction
Shipping Condition Dry Ice
Source EGFR residues 688-702, centered on Thr-693 (LVEPLTPSGEAPNQK)
Substrate Properties Peptide-Based Substrate
Target Entry MAPK11
Target Enzyme Serine/Threonine Kinases
Validated Kinases MAPK11
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For Research Use Only. Not for use in diagnostic procedures.