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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 11 Peptide

Catalog No. PV3670
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800 x 20 μL assays
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PV3670 800 x 20 μL assays
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Catalog No. PV3670 Supplier Invitrogen™ Supplier No. PV3670
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Description

Z'-LYTE Kinase Assay Kit - Ser/Thr 11 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine beta-casein A2, containing Ser-17.

Specifications

Product Line Z´-LYTE
Target Serine/Threonine Kinases
For Use With (Application) FRET
Assay Serine/Threonine Kinase Assay
Content And Storage Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Assay Entry Biochemical phospho-specific activity (no antibody)
Label or Dye Methylcoumarin
Product Type Kinase Assay Kit
Quantity 800 x 20 μL assays
Format 384-well plate
Sample Type bovine ß-casein A2 residues 14-21 (ESLSSSEE)
Excitation/Emission 405⁄465, 535
No. of Reactions 800 Reactions
References Eur. J. Biochemistry, 186, pp.459-464 (1989)
Readout End Point
Shipping Condition Dry Ice
Substrate Peptide-Based Substrate
Target Entry IKBKE, CSNK1D, CSNK2A1, FRAP1 (mTOR), CSNK1E, CSNK2A2, CSNK1A1
Validated Kinases CSNK1A1, CSNK1D, CSNK1E, CSNK2A1 , CSNK2A2, IKBKE, FRAP1 (mTOR)
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For Research Use Only. Not for use in diagnostic procedures.