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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide 
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
Supplier: Invitrogen™ PV3174
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.Spécifications
| Z´-LYTE | |
| FRET | |
| Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| Methylcoumarin | |
| 800 x 20 μL Assays | |
| porcine pyruvate liver kinase (LRRASLG) | |
| 800 Reactions | |
| End Point | |
| Peptide-Based Substrate | |
| AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5 |
| Serine/Threonine Kinases | |
| Serine/Threonine Kinase Assay | |
| Biochemical phospho-specific activity (no antibody) | |
| Kinase Assay Kit | |
| 384-well plate | |
| 405⁄465, 535 | |
| BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978) | |
| Dry Ice | |
| PRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA |
For Research Use Only. Not for use in diagnostic procedures.