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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide

Catalog No. PV3174
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800 x 20 μL Assays
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PV3174 800 x 20 μL Assays
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Catalog No. PV3174 Supplier Invitrogen™ Supplier No. PV3174
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Description

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.

Specifications

Product Line Z´-LYTE
Target Serine/Threonine Kinases
For Use With (Application) FRET
Assay Serine/Threonine Kinase Assay
Content And Storage Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Assay Entry Biochemical phospho-specific activity (no antibody)
Label or Dye Methylcoumarin
Product Type Kinase Assay Kit
Quantity 800 x 20 μL Assays
Format 384-well plate
Sample Type porcine pyruvate liver kinase (LRRASLG)
Excitation/Emission 405⁄465, 535
No. of Reactions 800 Reactions
References BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Readout End Point
Shipping Condition Dry Ice
Substrate Peptide-Based Substrate
Target Entry PRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA
Validated Kinases AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5
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For Research Use Only. Not for use in diagnostic procedures.