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Invitrogen™ ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit 
The ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit.
Supplier: Invitrogen™ K531000
Description
The ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO™ technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
- Stable expression
- Long-term experiments
- Accurate titer of functional virus
- Typically provides 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps
Key Features
- HiPerform™ WPRE and cPPT elements
- CMV promoter
- V5 epitope tag at C terminus
- Blasticidin selection
Kit Includes
- pLenti6.3⁄V5-TOPO™ TA Cloning™ Kit (K5315-20)
- ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
- 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
Specifications
| 1 kit | |
| TOPO™ TA Cloning Reagents (Box 1) • pLenti6.3⁄V5-TOPO™ (5-10 ng⁄μL) 20 μL, -20°C • pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 ug⁄μL) 20 μL, -20°C • 10X PCR Buffer 100 μL, -20°C • l dNTP Mix 10 μL, -20°C • Salt Solution 50 μL, -20°C • CMV Forward Primer (100 ng⁄μL) 20 μL, -20°C • V5 (C-term) Reverse Primer (100 ng⁄μL) 20 μL, -20°C • Control PCR Template (50 ng⁄μL) 10 μL, -20°C • Control PCR Primers (100 ng⁄μL) 10 μL each, -20°C • Sterile Water 1 mL One Shot™ Stbl3™ Chemically Competent E. coli (Box 2) • Stbl3™ Cells 21 x 50 μL, -80°C • pUC19 control DNA (10 pg⁄μL) 50 μL, -80°C • S.O.C. Medium 6 mL, -80°C Support kit • ViraPower™ Packaging Mix 195 μg, -20°C • Lipofectamine™ 2000 0.75 mL, +4°C • Blasticidin 50 mg, -20°C • 293 FT cells (3 x 106⁄mL) 1 mL, liquid nitrogen |
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| HiPerform™, TOPO™, ViraPower™ | |
| Lentiviral Expression Kit |
For Research Use Only. Not for use in diagnostic procedures.