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Gibco™ Viral Production Cells 2.0

Catalog No. p-7232453
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1 mL
6 x 1 mL
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A49784 1 mL
A51218 6 x 1 mL
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Catalog No. A49784 Supplier Gibco™ Supplier No. A49784
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Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

  • Adapted to the chemically defined Viral Production Medium and maintained in suspension culture in that medium
  • Highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
  • Supplied frozen in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
  • Should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
  • Clonal, 293F-derived, high-producer cell line
  • Absence of SV40 large T antigen
  • Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
  • Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
  • Robust scalability and passage stability

Specifications

Cell State Frozen
Concentration 1 x 107 cells/mL
Content And Storage 1 cryovial, store in vapor phase liquid nitrogen
Cell Line Viral Production Cells 2.0
Cell Type Kidney (Embryonic)
Culture Type Suspension Cell Culture
Form Frozen
Species Human
Format 1 Vial
For Use With (Application) AAV Production
Media Recommendation Gibco Viral Production Medium
No. of Cells 1 x 107 cells
Product Line AAV-MAX
Product Type Viral Production Cells
Quantity 1 mL
Shipping Condition Dry ice for domestic shipment; liquid nitrogen for international shipment
Source Human
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For Research Use Only. Not for use in diagnostic procedures.