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Gibco™ Viral Production Cells 2.0 DFS Item

Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

Supplier:  Gibco™ A51218

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Catalog No. A51218


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Description

Description

Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

  • Adapted to the chemically defined Viral Production Medium and maintained in suspension culture in that medium
  • Highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
  • Supplied frozen in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
  • Should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance
  • Clonal, 293F-derived, high-producer cell line
  • Absence of SV40 large T antigen
  • Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
  • Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
  • Robust scalability and passage stability
Specifications

Specifications

Frozen
6 cryovials, store in vapor phase liquid nitrogen
Kidney (Embryonic)
Frozen
6 Vials
Gibco Viral Production Medium
AAV-MAX
6 x 1 mL
Human
1e7 cells/mL
Viral Production Cells 2.0
Suspension Cell Culture
Human
AAV Production
1 x 107 cells
Viral Production Cells
dry ice for domestic shipment; liquid nitrogen for international shipment
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For Research Use Only. Not for use in diagnostic procedures.