missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. 758989782

Invitrogen™ Vimentin Monoclonal Antibody (V9), Alexa Fluor™ 420

Catalog No. 758989782
Change view
Click to view available options
Quantity:
100 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
758989782 100 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 758989782 Supplier Invitrogen™ Supplier No. 758989782
Only null left

Description

Mouse Monoclonal Antibody

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils. This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin. Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P). Applications Tested: This V9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 2.5 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific backg...

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

Antigen Vimentin
Applications Immunohistochemistry (Paraffin), Multiplex Immunohistochemistry
Classification Monoclonal
Clone V9
Concentration 0.2 mg/mL
Conjugate Alexa Fluor 420
Formulation PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2
Gene Vim
Gene Accession No. P08670
Gene Alias cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; Hel113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
Gene Symbols Vim
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7431
Target Species Human
Content And Storage 4°C, store in dark
Product Type Antibody
Form Liquid
Isotype IgG1 κ
Show More Show Less