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Applied Biosystems™ Veriti™ Thermal Cycler, 96-well Fast

Catalog No. 4375305
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Warranty:
5 years of total service coverage with instrument rapid exchange
Limited Warranty
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4375305 Limited Warranty
A27924 5 years of total service coverage with instrument rapid exchange
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Catalog No. 4375305 Supplier Applied Biosystems™ Supplier No. 4375305

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

The Applied Biosystems Veriti Thermal Cycler delivers the proven reliability of Applied Biosystems instruments. With the added control of VeriFlex technology, you have six independent temperature blocks that provide precise control over your PCR optimization. The Veriti 96-Well Fast Thermal Cycler is equipped with a Fast 0.1-mL block configuration.

The Applied Biosystems Veriti Thermal Cycler delivers the proven reliability of Applied Biosystems instruments. With the added control of VeriFlex technology, you have six independent temperature blocks that provide precise control over your PCR optimization. Additionally, the color touch screen simplifies setup and use of the Veriti Thermal Cycler. Optional setups for fast or standard PCR methods provide with flexibility to shorten your PCR cycling times. The Veriti 96-Well Fast Thermal Cycler is equipped with a Fast 0.1-mL block configuration.

Features include:

  • Innovative VeriFlex Blocks allow for precise PCR optimization and enable you to run up to six independent assays
  • Fast 0.1-mL block format is compatible with many downstream applications
  • Standard and fast enabled cycling to address your current and future PCR needs
  • Easy-to-use graphical interface (6.5 inch VGA Touch Screen)
  • Convenient protocol transfer from one Veriti Thermal Cycler to another using USB
  • Reduced PCR cycling time when using faster ramp rates
  • Optional VeritiLink Remote Management Software provides you with peace of mind, allowing you to remotely manage more than 50 cyclers from your PC or internet-compatible phone

VeriFlex Blocks: Enhanced PCR Functionality

The Veriti Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality. Six separate peltier blocks provide maximal versatility and flexibility and offer two key benefits: 1) PCR Optimization: each block can be set with a specific temperature which is ideal for precise control over PCR optimization 2) Run more experiments: the six peltier blocks can also be used to run up to six different annealing temperatures in the same run. With this enhanced functionality, the Veriti Thermal Cycler with VeriFlex blocks is the ideal thermal cycler for any lab.

Control at your fingertips

The powerful, yet simple to operate user interface on the Veriti system is driven by the 6.5 inch (16.51 cm) VGA color touch screen. The large screen allows for easy viewing of your temperature profiles. Additionally, the large navigation buttons put programming of the Veriti Thermal Cycler at your finger tips. Setup and navigation of the Veriti Thermal Cycler does not require the use of a stylus or mouse.

Fast setup, fast results

The Veriti system has two different options for method navigation. For quick setup, you may select one of the pre-programmed methods to run. If you prefer to program your own methods, simply touch the step you would like to setup and then use the keypad to enter thermal cycling steps. After completing a setup, you have the choice of saving the method to the instrument or to a USB memory stick.

Control your time: Go fast when you want

With the Veriti Thermal Cycler you get two thermal cyclers in one, a standard thermal cycler with its better than gradient feature, and a fast enabled thermal cycler. So whether you have a current need to run fast PCR and shorten your PCR cycling times, or a possible future application need for fast PCR, the Veriti Thermal Cycler provides you with the flexibility to go fast when you want.

Applied Biosystems PCR plastics

Applied Biosystems PCR plastics have been designed and validated to work with our thermal cyclers for more than 25 years. Options for every format and throughput need are available to ensure consistent, precise results for any experiment. To find the best match please visit our Plastics Selection Guide.

Specifications

Format 0.1 mL Tubes, 96-well Plate
Thermal Accuracy ±0.25°C (35°C to 99.9°C)
Sample Ramp Rate ±4.25°C/sec.
Temperature Range (Metric) 4°C to 99.9°C
Reaction Volume Range 10 to 30 μL
Content And Storage Veriti 96-well Fast Thermal Cycler, limited warranty
High-throughput Compatibility High-throughput Compatible
For Use With (Equipment) Veriti™ Thermal Cycler
Thermal Uniformity <0.5°C (20 sec. after reaching 95°C)
Reaction Speed Fast, Standard
Dimensions 19.1 x 9.3 x 9.6 in. (48.5 x 23.7 x 24.5 cm)
Capacity 96-well, 0.1 mL Tubes
Voltage 100/240 V
Product Line Veriti
Peak Block Ramp Rate 5.0°C/sec.
Display Type 6.5 in VGA 32 k Color Touchscreen
Product Type Thermal Cycler
Warranty Limited Warranty
Thermal Range 0°C to 100°C
For Use With (Application) PCR
Programs Auto re-start (after Power Outages), Program Overwrite Protection
User Interface USB, Wi-Fi, Ethernet
Max. Ramp Rate 5°C/sec. (Block), 4.25°C/sec. (Sample)
No. of Programs >500
Quantity 1 Instrument
Memory USB and On-board
Weight 25 lb. (11.4 kg)
Block Configurations 96-well, 6-Zone VeriFlex Block
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Frequently Asked Questions (FAQs)

Why are my PCR tubes crushed/deformed after my PCR run?

The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:

Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.

Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
What should I do if my Veriti Thermal Cycler fails the temperature non-uniformity test?

If the test fails:

1.Verify that the:
-Calibration of the TVK is up-to-date.
-Probe tips are not cracked or damaged.
2.Dispense 2 to 3 drops of mineral oil onto a cotton swab. Use the swab to apply a thin film of oil to the cone surface of each probe to improve heat transfer.
3.Repeat the steps in “Before You Run 96-Well Instrument Tests” from the Multiprobe Temperature Verification Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf).
4.Verify that the:
-Probes are seated securely in the appropriate test wells.
-Cables are connected securely to the ports.
-Ribbon cable lies flat under the thermal cutout and is not crimped.
-Heated cover is closed and locked down.
5.Repeat the steps in “Run the Test” from the Multiprobe Temperature Verification Kit User Guide (http://tools.thermofisher.com/content/sfs/manuals/4460108A.pdf). Please note, if the test fails again, contact us at instrumentservices@thermofisher.com.

My tubes are melting. What is wrong?

This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).

Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)

How do I perform the temperature verification on the Veriti Thermal Cycler?

Please refer to the guidelines in this protocol (https://tools.thermofisher.com/content/sfs/manuals/cms_070449.pdf) specific to the Veriti Thermal Cycler.

How do I program an infinite hold in the Veriti Thermal Cycler?

To add an infinite hold, select the infinity symbol for program.

How do the VeriFlex Blocks work on the Veriti Thermal Cycler?

The Veriti Thermal Cycler with a 96-well block (Fast and Standard) features innovative VeriFlex Blocks for enhanced PCR functionality. Six separate Peltier blocks provide maximal versatility and flexibility and offer two key benefits:
1. PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2. Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

What is the Auto Delta feature on the Veriti Thermal Cycler?

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
What is the max block ramp rate of the Veriti Thermal Cycler?

The 96-Well Block has a 3.9 degrees C/sec max block ramp rate, the 96-Well Fast Block has a 5.0 degress C/sec max block ramp rate, the 384- Well Block has a 3.7 degrees C/sec max block ramp rate, and the 60-Well Block has a 3.3 degrees C/sec max block ramp rate.
What types of plastics can I use with the Veriti Thermal Cycler?

The plates and plastics will be specific to the block type. Choose the appropriate block type below for a list of the compatible tubes, plates, and films.

-Veriti 60-Well
-Veriti 96-Well 0.1 mL
-Veriti 96-Well 0.2 mL
-Veriti 384-Well

What types of blocks are available on the Veriti Thermal Cycler?

The Veriti Thermal Cycler is available in four formats:
Veriti 96-Well Thermal Cycler (a 0.2 mL or 0.1 mL)
Veriti 384-Well Thermal Cycler
Veriti 60-Well Thermal Cycler Veriti 96-Well Fast Thermal Cycler

Have you tested the Veriti and VeritiPro Thermal Cyclers in low humidity conditions (below 20%)?

The recommended conditions are determined to ensure no static discharge (low humidity) occurs.
Recommended Conditions:
- Veriti: 20-80% humidity
- VeritiPro: 15-80% humidity
We have not tested Veriti and VeritiPro thermal cyclers in low humidity, below 20%, therefore we cannot recommend using them in those conditions.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

For Research Use Only. Not for use in diagnostic procedures.