Invitrogen™ ULYSIS™ Alexa Fluor™ Nucleic Acid Labeling Kit

Description

ULYSIS nucleic acid labeling kits provide a unique method for attaching a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast - just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.

Features include:

• Labeling reaction complete in as little as 15 minutes
• Available in several Alexa Fluor dye colors
• Non-enzymatic labeling—avoids the potential biases and limitations associated with enzymatic labeling methods
• Flexibility—compatible with a wide range of experimental conditions and protocols
• High sensitivity—provides strong fluorescence signals, enabling detection of low-abundance targets

Reliable labeling with the Universal Linkage System

This series of ULYSIS kits was developed to enable rapid and simple coupling of Alexa Fluor dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), makes use of a platinum dye complex that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.

Labeling is fast and easy

The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS complex can be accomplished with a simple spin-column procedure. DNA longer than ∼1,000 base pairs require a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.