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Invitrogen™ Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acetate)

Catalog No. T34078
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T34078 60 slides
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Catalog No. T34078 Supplier Invitrogen™ Supplier No. T34078
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Description

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells.

Tubulin Tracker Green is an uncharged, non-fluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form, inhibiting it from escaping the live cells. TubulinTracker Green reagent utilizes Oregon Green 488 dye, which absorbs and emits optimally at 494 nm and 522 nm, respectively, and fluorescence can be visualized with standard GFP/FITC filter settings using almost any fluorescent imaging instrument. It can be multiplex with blue, orange, red, deep-red, and near-IR fluorophores.

Specifications

Content And Storage Contains sufficient material for at least 60 slides at 1 mL / slide and 1 x working concentration.

1 x 20 μg Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate)

1 x 1 mL Pluronic F-127, 20% solution in DMSO
Excitation Wavelength Range 494/522
Dye Type Oregon Green™ 488
Quantity 60 slides
Product Line TubulinTracker
Shipping Condition Approved for shipment on Wet or Dry Ice
Label Type UV-Vis Labels
Product Type Tubulin Tracker
SubCellular Localization Tubulin

Frequently Asked Questions (FAQs)

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.
With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

For Research Use Only. Not for use in diagnostic procedures.