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Invitrogen™ TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System
Description
Includes
One bottle of TRIzol LS Reagent 100 mL and one bag of 100 Phasemaker Tubes
This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes. The TRIzol Reagent offers superior lysis capability, even with difficult sample types. Invitrogen™ Phasemaker Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol mix from the organic phase underneath, making very easy to pipette off the upper phase containing the RNA.
The polymer works on density gradient, as it is heavier than the aqueous phase, but lighter that the organic phase; after centrifugation it positions itself between these two layers. It creates a tight seal between the two phases, making it very easy to pipette off the aqueous phase without the risk of carrying over contaminants from the organic layer. Because this, Invitrogen Phasemaker Tubes allow you to recover the whole aqueous phase, increasing yield up to 30%. It is an inert polymer that does not interfere with downstream experiments such as PCR. It works with fresh or frozen samples (tissue or blood). It also works with tissue samples treated previously with RNAlater. Invitrogen Phasemaker Tubes come already pre-dispensed. The tube size is 2 mL.
• Complete System includes TRIzol and Phasemaker Tubes
• Phasemaker Tubes are pre-dispensed in 2 mL tubes
• Creates a tight seal between the aqueous and the organic phases
• Easier to pipette off the whole aqueous phase
• Increases yield up to 30%
• RNA integrity is not compromised
• The seal stays for longer periods of time, allowing to increase the number of samples per batch
• Inert, does not interfere with downstream experiments
Warranty
One year after receiving
Specifications
Specifications
| Content And Storage | • 100 mL TRIzol LS Reagent; room temperature • 100 Phasemaker Tubes; room temperature |
| Isolation Technology | Organic Extraction |
| Purification Time | 1 hr. |
| Elution Volume | 20 to 50 μL |
| Sample Type | Liquid Samples (e.g. Serum) |
| Final Product Type | Total RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling |
| Quantity | 100 Preps |
| Shipping Condition | Room Temperature |
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Frequently Asked Questions (FAQs)
The polymer in Phasemaker Tubes is inert and heat-stable, and does not affect standard downstream applications performed after RNA extraction.
Yes you can. As RNAlater incorporates salts into the sample, the salt content can increase the density of the sample mix, so we recommend adding RNAse-free water (50-100 µL) to the sample mix.
Phasemaker Tubes work on the principle of density gradient centrifugation. Phasemaker Tubes contain a thick liquid polymer that is heavier than the aqueous phase of the TRIzol Reagent mix, but lighter than the organic phase, so after centrifugation, the polymer positions itself between these two layers and forms an exclusive physical barrier. It separates and completely isolates the upper aqueous phase from the organic phase underneath, making it very easy to aspirate the upper phase containing the RNA.
Phasemaker Tubes work exclusively with TRIzol Reagent (and TRIzol LS Reagent ). As other similar reagents may have different density, Phasemaker Tubes may not work properly.
No. Phasemaker tubes are only validated for RNA extraction.
For Research Use Only. Not for use in diagnostic procedures.