Invitrogen™ Triiodothyronine (T3) Competitive ELISA Kit

ELISA

The Triiodothyronine (T3) ELISA quantitates T3 in serum, plasma, urine, dried fecal extracts, or cell culture medium. Principle of the method The Triiodothyronine (T3) Competitive ELISA research-use-only kit is designed to quantitatively measure T3 present in extracted serum and plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples. A T3 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.