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  5. Invitrogen™ TOP10 Electrocomp™ Kit

Invitrogen™ TOP10 Electrocomp™ Kit

Catalog No. C66455
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5 x 100 μL
10 x 100 μL
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C66455 5 x 100 μL
C66411 10 x 100 μL
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Catalog No. C66455 Supplier Invitrogen™ Supplier No. C66455
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Description

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids. 

The TOP10 genotype is similar to the DH10B strain and features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Notes
Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

Find the strain and format that fits your needs
• TOP10 is available in chemically competent and electrocompetent cell formats.
• Choose MultiShot competent cells for high-throughput cloning applications.

Specifications

Product Type Electrocompetent Cells
Contains F' Episome No
Improves Plasmid Quality Yes (endA1)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 1010 cfu/μg)
Content And Storage • 5 x 100μL TOP10 Electrocomp E. coli
• 50 μL pUC19 vector (10 pg/μL)
Store at –80°C.

• S.O.C. Medium (15 mL)
Store at room temperature.
Antibiotic Resistance Bacterial Yes (Streptomycin)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Blue/White Screening Yes (lacΔM15)
High-throughput Compatibility Low
Plasmid May be used for plasmids >20 kb
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
Shipping Condition Dry Ice
T1 Phage - Resistant (tonA) No
Species E. coli (K12)
Format Tube
Product Line Electrocomp
Quantity 5 x 100 μL
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For Research Use Only. Not for use in diagnostic procedures.