PeproTech TNFR2 (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hsTNF Receptor II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor II (500-P168) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hsTNF Receptor II. Western Blot: To detect hsTNF Receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF Receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P168BT-1MG will be provided as 2 x 500 μg

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.