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  6. Thermo Scientific™ TMT11-131C Label Reagent

Thermo Scientific™ TMT11-131C Label Reagent

Catalog No. pia37725
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Quantity:
1 x 5 mg
1 x 5 mg (per tag)
3 x 0.8 mg
3 x 0.8 mg (per tag)
8 x 40 μg​ (per tag), 80 reactions
Catalog No. Quantity
PIA37725 3 x 0.8 mg (per tag)
PIA34807 1 x 5 mg
PIA37724 3 x 0.8 mg
PIA34808 1 x 5 mg (per tag)
FERA58333 8 x 40 μg​ (per tag), 80 reactions
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Catalog No. PIA37725

Supplier: Thermo Scientific™ A37725

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Description

Includes

TMT10plex Isobaric Mass Tag Labeleing Reagent Set plus TMT11-131C

Tandem mass tag (TMT) reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry.

Tandem mass tag (TMT) reagents are specially designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT11-131C tag structure is identical to the other TMT10pex tags, but the TMT11-131C mass reporter is labeled with only 13C isotopes. The addition of the TMT11-131C tag enables separation of the 131-mass reporter ion of the original TMT10plex reagents into N and C variants, creating an 11th channel for relative quantitation using high resolution Orbitrap MS instruments.

The advantages of TMT label reagents include increased multiplexed relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents plus TMT11-131C is ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or to improve statistics on biological replicates.

Features of this TMT11-131C Label Reagent include:
• Multiplex—concurrent MS analysis of up to 11 samples derived from cells, tissues, or biological fluids
• Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
• Efficient—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
• Compatible—optimized for use with high resolution Thermo Scientific Orbirtrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3

TMT11-131C is available as a standalone tag (Cat. No. A34807, A37724) or as part of this TMT10plex Isobaric Label Reagent Set plus TMT11-131C. When combined with the industry-leading, high resolution Orbitrap instruments and software, TMT reagents provide integrated total solutions for quantitative protein expression analysis.

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Isobaric Label Reagent
Product Line TMT10plex
Content And Storage 3 sets of 11 vials, store at –5 to –30°C
Label or Dye Mass Isotopes of Amino Acids
Shipping Condition Dry Ice
Starting Material Peptides
Final Product Type Peptide
Detection Method Mass Spectrometry
Workflow Step Peptide Labeling
Quantity 3 x 0.8 mg (per tag)
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