Invitrogen™ Thyroxine (T4) Competitive ELISA Kit

ELISA

The Thyroxine (T4) ELISA quantitates Thyroxine (T4) in serum, plasma, urine, dried fecal extracts, or cell culture medium. Principle of the method The Thyroxine (T4) Competitive ELISA research-use-only kit is designed to quantitatively measure T4 present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. The kit offers two standard curve ranges. For serum and plasma samples we recommend using 10 μL of standards or samples. The assay concentration range for T4 will be from 20 ng/mL to 0.625 ng/mL. For urine samples we recommend using 100 μL of standards or samples. Assay concentrations of T4 that range from 4 ng/mL to 0.0625 ng/mL can be measured. A T4 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A T4-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to T4 to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T4-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm. Rigorous validation