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Molecular Probes™ ThiolTracker™ Violet (glutathione detection reagent)

Catalog No. T10096
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5 Microplate(s)
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T10096 5 Microplate(s)
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Description

ThiolTracker™ Violet (glutathione detection reagent)

ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free thiols in the cell, ThiolTracker™ Violet can be used in estimating the cellular level of reduced glutathione.
ThiolTracker™ Violet offers the following;
  • 10X brighter than mBCL
  • UV or 405 nm excitation, 525 nm emission
  • Easy to use
  • Reproducible results
  • Can be fixed with aldehydes and permeabilized by Triton™ X-100 (0.5%)
  • Formaldehyde fixable and suvives detergent extraction
  • Can be used in multiplex assays including cytotoxicity studies
    Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection. ThiolTracker™ Violet can be used for Flow Cytometry, HCS imaging, and epifluorescent microscopy.

    • Specifications

    Color Violet
    Content And Storage

    Store at ≤–20°C, L protect material from long-term exposure to light; may be exposed to light for short periods of time, requires DMSO
    Excitation Wavelength Range 405⁄526
    Dye Type ThiolTracker™ Violet
    Format Tube(s), 96-well plate, Slide(s)
    For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
    Quantity 5 Microplate(s)
    Detection Method Fluorescence
    Emission Visible
    Form Lyophilized
    Product Line Molecular Probes, ThiolTracker
    Shipping Condition Room Temperature
    Label Type Other Labels or Dyes
    Product Type Glutathione Detection Reagent
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    Frequently Asked Questions (FAQs)

    Is ThiolTracker Violet a fluorogenic reagent?

    No. ThiolTracker Violet is fluorescent whether bound or unbound to a sulfhydryl group.

    Will ThiolTracker Violet bind to proteins as well as glutathione?

    Yes, ThiolTracker Violet will bind to any accessible sulfhydryl group (-SH; cysteine) whether it is on reduced glutathione or on the surface of a protein. It will not bind to sulfur bound via a disulfide bond (cystine) or ‘buried’ cysteines.

    For Research Use Only. Not for use in diagnostic procedures.