missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Assays
  5. Bioluminescence Assays and Reagents
  6. PI88241

Thermo Scientific™ pEF1α Tluc16-DD Vector for Luciferase Assays

Catalog No. PI88241
Change view
Click to view available options
Quantity:
10 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI88241 10 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI88241 Supplier Thermo Scientific™ Supplier No. 88241
Only null left

Description

Insert promoter sequences for genes of interest into Thermo Scientific™ Tluc16 Vectors to measure gene regulation using the intracellular TurboLuc™ 16 (Tluc16) luciferase reporter in luciferase assays.

The Thermo Scientific pMCS Tluc16 Vectors are multiple cloning site (MCS) vectors designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.

  • Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
  • Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through
  • Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
  • Optimized minimal core promoter (minP) and 5' UTR region for efficient expression of Tluc16 luciferase
  • Hygromycin resistance gene for use as selection marker to generate mammalian stable cell lines
  • Prepared pNF-κB, and pCRE Tluc16-DD Vectors are designed to monitor the activation of NFκB protein and cAMP-binding protein (CREB) in mammalian cells
  • Prepared pEF1α variety includes a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells
Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression. A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the MCS to minimize non-specific transcriptional read-through. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.

Specifications

Type Transcriptional Reporter Vector
Cloning Method Restriction Enzyme ⁄ MCS
Delivery Type Transfection
Quantity 10 μg
Content And Storage Store in freezer (-5 to -30°C).
Reporter Gene TurboLuc™16 Luciferase
For Use With (Application) Reporter Assays
Promoter EF-1α

Frequently Asked Questions (FAQs)

Can I use any luciferase vector with the TurboLuc Luciferase One-Step Glow Assay Kit?

We offer three vectors with commonly used promoters built into the vectors:

  • pEF1a-Tluc16-DD Vector (Human Elongation Factor-1 alpha; Cat. No. 88241)

  • pNFkBTluc16 DD Vector (Nuclear Factor-kappa B; Cat. No. 88246)

  • pCRE Tluc16 DD Vector (Carbapenem-resistant Enterobacteriaceae; Cat. No. 88247)


Vector-specific information can be found in the lot-specific Certificate of Analysis on the website
What options do you offer for luciferase vectors?

There are three vectors available for each expressed luciferase; pMCS, pCMV, and pTK. We recommend using pMCS to measure the activity of the reporter, and pCMV, and pTK as positive control vectors. For high level constitutive expression of Luciferase, we recommend using the CMV (Cytomegalovirus) promoter vector and for low level constitutive expression of luciferase, we recommend using the pTK (thymidine kinase) promoter vector.

What is the stability of the secreted luciferase?

Gaussia, Gaussia-Dura, and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours.
How quickly is luciferase secreted?

The earliest detectable signal will depend on the strength of the promoter. In general, with a strong promoter (such as CMV), significant signal over background can be seen in as little as 20 minutes. However, with a weaker inducible promoter, significant signal over background may take 1-2 hours after induction.
Do you still offer quantitative IP (qIP) Luciferase assay kits?

Our qIP Luciferase assay kits have been discontinued but we do carry epitope-tagged (HA or c-Myc) and Tluc (TurboLuc luciferase)-tagged mammalian expression vectors, and qIP protein interaction assay reagents for these assay kits.

For Research Use Only. Not for use in diagnostic procedures.