Efficient, gentle lysis and extraction of E.coli and other bacterial cells
$597.86 - $1,077.95
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.These easy-to-use cell lysis reagents are nonionic detergent-based solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. B-PER Complete Bacterial Protein Extraction Reagent is an all-inclusive formulation that combines the lysis reagent with lysozyme and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria. Traditional Tris-buffered and phosphate-buffered formulations without enzymes are also offered. All B-PER Reagents are compatible with downstream applications, such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay). Depending on the particular application, protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.