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  5. Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Catalog No. PI89822
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250 mL
500 mL
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Catalog No. Quantity
PI89822 500 mL
PI89821 250 mL
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Catalog No. PI89822 Supplier Thermo Scientific™ Supplier No. 89822
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Description

Includes

Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

  • An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
  • All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
  • This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
  • Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

  • Detergent in Tris buffer with Lysozyme and Universal Nuclease
  • Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
  • Recovery of large molecular weight proteins (high MW proteins)
  • Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
  • More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications

Specifications

Formulation Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Quantity 500 mL
Product Type Bacterial Protein Extraction Reagent
Volume (Metric) 500 mL
Content And Storage Store at 4°C.
Product Line B-PER

For Research Use Only. Not for use in diagnostic procedures.