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  6. Thermo Scientific™ TMT10plex™ Isobaric Label Reagents and Kits

Thermo Scientific™ TMT10plex™ Isobaric Label Reagents and Kits

Catalog No. PI90110
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Quantity:
1 x 0.8 mg (per tag), 10 Reactions
1 x 5 mg (per tag), 60 Reactions
3 x 0.8 mg (per tag), 30 Reactions
3 x 0.8 mg
8 x 0.2 mg
Product Type:
Isobaric Label Reagent Set
Isobaric Mass Tag Labeling Kit
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Catalog No. Quantity Product Type
PI90110 1 x 0.8 mg (per tag), 10 Reactions Isobaric Label Reagent Set
PI90111 3 x 0.8 mg (per tag), 30 Reactions Isobaric Label Reagent Set
PI90406 1 x 5 mg (per tag), 60 Reactions Isobaric Label Reagent Set
PI90309 8 x 0.2 mg Isobaric Label Reagent Set
PI90113 3 x 0.8 mg Isobaric Mass Tag Labeling Kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
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Catalog No. PI90110 Supplier Thermo Scientific™ Supplier No. 90110

Description

TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter.

TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter. Up to ten different peptide samples prepared from cells or tissues can be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass (i.e., TMT10 126-131 Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry.

The Tandem Mass Tag (TMT) Isobaric Label reagents and kits are specifically designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The TMT10plex label reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex reagents, but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter. The different isotopes result in a 10-plex set of tags that have mass differences in the reporter that can be detected using high resolution Orbitrap MS instruments.

Advantages of the TMT10plex label reagents include increased multiplex relative quantitation, increased sample throughput, and fewer missing quantitative values among samples. TMT10plex label reagents are ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or improving statistics on biological replicates.

Features of TMT10plex Isobaric Label reagent sets and mass tagging kits:

  • Powerful—multiplexed MS analysis increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues, or biological fluids
  • Consistent—identical reagent structure and performance among TMTzero, TMTduplex, TMTsixplex, and TMT10plex reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets
  • Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
  • Efficient—amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
  • Compatible—optimized for use with high resolution Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3.

Applications:

  • Protein identification and quantitation from multiple samples of cells, tissue, or biological fluids
  • Protein expression profiling of normal vs. disease states or control vs. treated
  • Quantitative analysis of proteins for which no antibodies are available
  • Identification and quantitation of membrane and post-translationally modified proteins
  • Identification and quantification of hundreds to thousands of proteins in a single experiment

TMT Reagents are provided as standalone sets, a 96-well format, or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience, and reliability. When combined with the industry-leading, high resolution Thermo Scientific Orbitrap instruments and software, TMT10plex reagents provide integrated total solutions for quantitative protein expression analysis.

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Isobaric Label Reagent Set
Product Line TMT10plex
Sufficient For 10 Reactions
Content And Storage Sufficient for: One 10-plex (1 x 10-way) experiment
• TMT10-126 Label Reagent, 1 x 0.8 mg
• TMT10-127N Label Reagent, 1 x 0.8 mg
• TMT10-127C Label Reagent, 1 x 0.8 mg
• TMT10-128N Label Reagent, 1 x 0.8 mg
• TMT10-128C Label Reagent, 1 x 0.8 mg
• TMT10-129N Label Reagent, 1 x 0.8 mg
• TMT10-129C Label Reagent, 1 x 0.8 mg
• TMT10-130N Label Reagent, 1 x 0.8 mg
• TMT10-130C Label Reagent, 1 x 0.8 mg
• TMT10-131 Label Reagent, 1 x 0.8 mg

Store at –20°C.
Label or Dye Tandem Mass Tag™ (TMT) Labels
Starting Material Cell Lysate
Final Product Type Protein PEPTIDE
Detection Method Mass Spectrometry
Workflow Step Protein Labeling
Quantity 1 x 0.8 mg (per tag), 10 Reactions
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