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Thermo Scientific™ MuSeek Index Set 1, Illumina compatible

Catalog No. FERK1551
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24 Dual Indices (4 x 6)
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FERK1551 24 Dual Indices (4 x 6)
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Catalog No. FERK1551 Supplier Thermo Scientific™ Supplier No. K1551
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Description

Enables pooling of up to 24 dual-indexed libraries in one sequencing run

MuSeek Index Set 1, Illumina compatible, is designed for generation of indexed genomic DNA libraries for sequencing on the Illumina MiSeq™ and HiSeq™ instruments. The set provides 4 indexed P5 adapters and 6 MuSeek indexed P7 adapters. This enables pooling of up to 24 dual-indexed libraries and conducting multiplexed sequencing analysis. The MuSeek Index Set 1, in combination with MuSeek Library Preparation kit, Illumina compatible, allows fast and simple indexed library construction from 50ng of sample DNA, eliminating the need for physical shearing, end conversion and adapter ligation steps.

Highlights

  • Enables sequencing of up to 24 dual-indexed fragment libraries in a single sequencing run.
Applications
  • Indexed DNA fragment library preparation for next generation sequencing.
Includes
  • MuSeek Indices M501-4
  • MuSeek Indices M701-6

Specifications

Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o Tango™ CMKLR1-bla U2OS DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70μg
o Solution D
o Tango™ CMKLR1-bla U2OS Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The Tango™ CMKLR1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Format Kit
Sample Type DNA
Sequencing Type Genome and DNA Sequencing
For Use With (Equipment) Illumina™ HiSeq 2500, Illumina ™ MiSeq
For Use With (Application) Sequencing
Libraries Fragment Library
Product Line MuSeek
Product Type MuSeek Index Set 1
Quantity 24 Dual Indices (4 x 6)
Workflow Step Library Generation
Form Solution
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Frequently Asked Questions (FAQs)

Is MuSeek suitable with ssDNA?

MuSeek is not suitable for ssDNA, as MuA transposase is not able to fragment ssDNA. To use MuSeek, ssDNA should first be converted into dsDNA.
Can I use MuSeek to construct NGS library from a PCR product?

Yes. However for construction of NGS library from PCR amplicons in fragmentation reaction, do not use PCR products shorter than 300 bp. Due to intrinsic features of the transposon technology, a approximately 50 bp drop off is expected in sequencing coverage from each distal end of the amplicon sequence. This can be averted by designing your amplicons to be approximately 100 bases larger than the desired sequencing insert.
Can I use lower than recommended sample amount for MuSeek fragmentation reaction?

No, the recommended sample DNA input should be followed strictly. The MuSeek fragmentation reaction is optimized to yield optimal fragment library lengths at recommended input.
Is MuSeek compatible with RNA library preparation workflow?

We do not supply specific MuSeek kits for NGS library preparation from RNA samples. However, MuSeek is compatible with double-stranded cDNA substrate.
What is the difference between the ClaSeek technology and MuSeek technology?

The ClaSeek technology uses a classical NGS library preparation approach where physically fragmented DNA is end-repaired and platform-specific adapters are added through ligation reaction. MuSeek transposase-based technology simultaneously fragments intact DNA and adds MuA transposon derived DNA sequence which is further used for PCR-based adapter addition step.
What is the sample input requirement for the MuSeek protocol?

MuSeek provides a protocol for NGS library construction from 50 ng or 100 ng sample DNA.
Do I need to perform fragmentation of DNA prior to the MuSeek protocol?

No. The MuSeek protocol does not require any physical fragmentation of the sample DNA. The MuSeek Library Preparation Kit utilizes a transposon-based technology that allows enzymatic fragmentation and simultaneous tagging of the target DNA, eliminating the need for separate shearing, DNA end repair, and adaptor ligation steps.
How does MuSeek DNA sample preparation technology work?

MuSeek technology utilizes the MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step, the platform-specific adaptors are added using Thermo Scientific Phusion High-Fidelity DNA polymerase.
What Illumina sequencing platforms are compatible with MuSeek?

NGS libraries prepared using MuSeek Library Preparation Kit (Illumina compatible) is suitable for sequencing on Illumina MiSeq and Illumina HiSeq instruments.
Does MuSeek technology support indexing? How many indices does MuSeek support?

Yes, Thermo Scientific MuSeek Library Preparation Kit (Illumina compatible) in combination with MuSeek Index Set 1 enables indexing and pooling of up to 24 libraries in one sequencing run. MuSeek Library Preparation Kit for Ion Torrent with MuSeek Barcode Set 1 (Ion Torrent compatible) enables up to 16 fragment libraries.

For Research Use Only. Not for use in diagnostic procedures.