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  5. Invitrogen™ Human TFPI ELISA Kit

Invitrogen™ Human TFPI ELISA Kit

Catalog No. EHTFPI
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96 Tests
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Catalog No. Quantity
EHTFPI 96 Tests
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Catalog No. EHTFPI Supplier Invitrogen™ Supplier No. EHTFPI

Description

ELISA

The Human TFPI ELISA quantitates Hu TFPI in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TFPI. Principle of the method The Human TFPI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tissue factor pathway inhibitor (TFPI), also known as lipoprotein-associated coagulation inhibitor, is a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. TFPI is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. TFPI inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop.

Specifications

Accession Number P10646
Assay Range 5-1800 pg/mL
Assay Sensitivity 5 pg/mL
Calibration Range 5 pg/mL to 1800 pg/mL
Content And Storage Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
Assay Solid-phase sandwich ELISA
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Protein Family Proteases Peptidases & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA antibody pair detects human TFPI. Other species not determined.
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias A630013F22Rik; anti-convertin; AW552122; EPI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; OTTHUMP00000163472; OTTHUMP00000205431; TFI; TFPI; TFPI1; Tissue factor pathway inhibitor; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)A630013F22Rik; anti-convertin; AW552122; EPI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; OTTHUMP00000163472; OTTHUMP00000205431; TFI; TFPI; TFPI1; Tissue factor pathway inhibitor; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); EPI; LACI; TFI1; TFPI
Gene ID (Entrez) 7035
Gene Symbol TFPI
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases TFPI, Extrinsic pathway inhibitor, EPI, Lipoprotein-associated coagulation inhibitor, LACI
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Other Research
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Synonym EPI, LACI, TFI1, TFPI
Target TFPI
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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