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Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent

Catalog No. PI78510
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PI78510 500 mL
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Catalog No. PI78510 Supplier Thermo Scientific™ Supplier No. 78510
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Description

Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.

Perform mild extraction of total protein from tissue samples with Thermo Scientific™ T-PER Tissue Protein Extraction Reagent.

This tissue cell lysis reagent utilizes a proprietary detergent in 25mM bicine and 150mM sodium chloride (pH 7.6) to maximize the efficiency of protein solubilization from mammalian tissue samples by homogenization. The simple composition is compatible with additives such as protease inhibitors, salts, reducing agents and chelating agents, providing versatility for many different sample types and lysis applications. Cell lysates prepared with T-PER Reagent are directly compatible with reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures.

Highlights:

  • Simple procedure – just homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell/tissue debris

  • Mild and convenient – detergent is dialyzable for quick and easy removal; can be used with additional components (e.g., protease inhibitors, salts, reducing agents, chelating agents, etc.)

  • Flexible – lysate may be used for reporter assays, protein kinase assays, immunoassays and/or protein purifications

  • Compatible – the lysate is compatible with standard protein assays such as Thermo Scientific Pierce™ Coomassie Plus™ (Bradford) Protein Assay

Specifications

Quantity 500 mL
Format Liquid
Product Type Tissue Protein Extraction Reagent
Volume (Metric) 500 mL
Content And Storage Store at room temperature.
Product Line T-PER

Frequently Asked Questions (FAQs)

Which protein assays are the T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) compatible with?

The T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) is compatible with standard protein assays such as Pierce BCA Protein Assay (Cat. No. 23225) and Coomassie Plus (Bradford) Assay Kit (Cat. No. 23236).
What is the shelf life of T-PER Tissue Protein Extraction Reagent (Cat. No. 78510)?

T-PER Tissue Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
Can you provide the shelf-life for the T-PER Tissue Protein Extraction Reagent?

The T-PER Tissue Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
I only want to buy one reagent for extraction of protein from both mammalian cells and tissues. Will M-PER Mammalian Protein Extraction Reagent work for protein extraction from tissue samples?

For extraction of protein from tissue samples as well as some difficult-to-lyse mammalian cells, we recommend using T-PER Tissue Protein Extraction Reagent (Cat. No. 78510) with homogenization.
What is the basic extraction protocol using T-PER Tissue Protein Extraction Reagent?

Homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell or tissue debris, and collect supernatant. For example, use a ratio of 100 mg of tissue to 2 mL T-PER Reagent. Use a smaller volume of reagent if a more concentrated protein extract is required.

What are the storage and shipping conditions for T-PER Tissue Protein Extraction Reagent?

T-PER Tissue Protein Extraction Reagent is shipped at ambient temperature. We recommend storing the product at room temperature.
Which assays can be tested with a T-PER Tissue Protein Extraction Reagent lysate?

The prepared cell lysate may be used for immunoassays (Western blots, ELISAs, RIAs), reporter assays (luciferase, β-galactosidase, chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, tyrosine kinase), and protein purification.
Can I still use the T-PER Tissue Protein Extraction Reagent if I do not have a Dounce homogenizer?

Yes, the reagent can be added directly to tissue that has been prepared using a mortar and pestle or any other mechanical means.
How should the tissue be prepared when using the T-PER Tissue Protein Extraction Reagent?

Fresh tissue is always the best though frozen tissue can also be used with the reagent. Paraffin embedded or preserved tissue may present a problem for the lysis reagent, since the reagent does not permeate the cells in the tissue uniformly, so protein yield will be reduced. Mechanical disruption of the tissue is still required for all types of tissue. A hand-held homogenizer may be used, or the tissue may be frozen in liquid nitrogen and then ground.
How can I quantify my protein following extraction with T-PER Tissue Protein Extraction Reagent?

The T-PER Tissue Protein Extraction Reagent is compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236). It is also compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660) after a 1:2 dilution.
Can I separate out the nuclear and cytoplasmic proteins using T-PER Tissue Protein Extraction Reagent?

No. Both phases will be extracted together. We recommend Mem-PER PLUS Membrane Protein Extraction Kit (Cat. No. 89842) and NE-PER Nuclear and Cytoplasmic Extraction Reagents (Cat. No. 78833) to isolate membrane and nuclear proteins, respectively from tissue samples
Which tissue types have been tested with T-PER Tissue Protein Extraction Reagent?

Mouse heart, liver, kidney, and brain tissues have been tested with the T-PER Tissue Protein Extraction Reagent. This product performs exceptionally well for tumor growth factor and insulin growth factor extraction. T-PER Reagent was validated for protein yield in both fresh and frozen tissue. Additionally, T-PER Reagent was shown to efficiently extract target proteins from various cellular compartments such as plasma membrane (Na-K+ ATPase, pan-cadherin), cytoplasm (HSP90, MAPK), mitochondria (COXIV), and nucleus (HDAC1), and tissue specific markers from brain (synaptophysin) and heart tissue (Troponin T Cardiac Isoform).
What are the characteristics of T-PER Tissue Protein Extraction Reagent?

T-PER Tissue Protein Extraction Reagent is provided as a 500 mL volume, and contains a mild zwitterionic dialyzable detergent in 25 mM Bicine, 150 mM sodium chloride at pH 7.6. The sodium chloride in T-PER Reagent was added to the formulation to make the reagent more compatible with downstream assays normally carried out from tissue preparations. We recommend adding Halt Protease Inhibitor Cocktail (Cat. No. 87785) to the T-PER Reagent just before use.
Why should I use T-PER Tissue Protein Extraction Reagent (Cat. No. 78510)?

T-PER Tissue Protein Extraction Reagent enables mild extraction of total protein from tissue samples. T-PER Reagent was designed for use with all tissues, independent of mechanical extraction methods. The mild detergent enhances solubilization of proteins without compromising function, and the neutral pH and salt concentration of this reagent enable efficient extraction from cellular compartments yielding homogeneous lysates.

What lysis buffers do you offer for mammalian cells?

Please see the various lysis buffers we offer (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/cell-lysis-fractionation/cell-lysis-total-protein-extraction.html) for total protein extraction from mammalian cells.

For Research Use Only. Not for use in diagnostic procedures.