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Invitrogen™ SYTOX™ Green Ready Flow™ Reagent

Catalog No. R37168
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R37168 120 Assays
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Catalog No. R37168 Supplier Invitrogen™ Supplier No. R37168
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Description

SYTOX™ Green Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays.

  • A bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays
  • It is excitable at 488 nm with an emission profile similar to FITC, and has been tested in multiple cell lines
  • Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze
  • As a cell-impermeant dye, SYTOX Green enters the cell upon loss of membrane integrity and binds to DNA, thereby acting as a counterstain that can be analyzed when excited
  • SYTOX Green reagent can be used in both mammalian and bacterial cells
  • It is often included in multiplex assays

Specifications

Cell Permeability Impermeant
Color Green
Content And Storage Includes 2 dropper bottles, store at room temperature.
Description SYTOX™ Green Ready Flow™ Reagent
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Reagent
Dye Type SYTOX™ Green Ready Flow™
Emission 523
Excitation Wavelength Range 504 nm
Form Solution
Format Bottle(s)
Product Line Ready Flow, Sytox
Quantity 120 Assays
Solubility DMSO (Dimethylsulfoxide)
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Frequently Asked Questions (FAQs)

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.
I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.
Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.