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  6. Invitrogen™ SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells

Invitrogen™ SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells

Catalog No. S11380
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Catalog No. S11380 Supplier Invitrogen™ Supplier No. S11380
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Description

SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes and yet does not cross the membranes of live cells.

With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells. After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration.

Features of SYTOX Deep Red Nucleic Acid Stain include:
• Impermeant to live cells; permeant to dead or fixed cells
• Excitation/emission peak: 660/682 nm
• Use with Cy5 traditional filter set or 647 laser line
• Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria
• Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers

SYTOX Deep Red Nucleic Acid Stain is multiplexable with blue, green, orange, red, and near IR fluorophores, when compatible fluorescent filter/laser configurations are used. SYTOX Deep Red stain shows increased fluorescence with increasing concentrations of dsDNA, but does not show much affinity for RNA or ssDNA. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make SYTOX Deep Red stain a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers.

SYTOX Deep Red Nucleic Acid Stain had been used successfully in mono-layer cells, thin-sliced tissue, thick-sliced tissue, 3D cell cultures/spheroids, and bacteria. It is supplied at a 1 mM concentration in DMSO (∼2000X concentration). A concentration of 0.5 μM (1X) is recommended for most applications and cell types. In fluorescent imaging of various mammalian cells, a concentration range of 2.5 μM to 0.25 μM has been used. For viability testing by flow cytometry, a concentration of 1 μM to 10 nM has been used for Jurkat cells and a range of 4 μM to 0.5 μM has been used with E. coli.

Specifications

Color Deep Red
Content And Storage Store in freezer at -5°C to -30°C and protect from light.
Excitation Wavelength Range 660 nm
Dye Type Cell-Permeant
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope
Quantity 50 μL
Volume (Metric) 50 μL
Detection Method Fluorescence
Emission 682 nm
Product Line SYTOX
Shipping Condition Approved for shipment on Wet or Dry Ice
Product Type Nucleic Acid Stain
Sub Cellular Localization Nucleic Acids
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Frequently Asked Questions (FAQs)

Can I use SYTOX Deep Red Nucleic Acid Stain with a cell permeable nucleic acid stain for live/dead cell analysis?

Yes. For live cell staining, you may use any of the blue, green, or orange SYTO dyes or any of the blue fluorescent Hoechst stains.
Can I use SYTOX Deep Red Nucleic Acid Stain for cell cycle analysis?

We do not recommend using SYTOX Deep Red Nucleic Acid Stain for cell cycle analysis. Instead, we recommend using Vybrant DyeCycle Ruby Stain (Cat Nos. V10273, V10309).
How is SYTOX Deep Red Nucleic Acid Stain, for fixed/dead cells (Cat. Nos. S11380, S11381) different from SYTOX Red Dead Cell Stain (Cat No. S34859)?

Although they are both far red, cell impermeant nucleic acid stains, there is approximately a 20 nm difference in the excitation and emission maxima: SYTOX Deep Red Nucleic Acid Stain ex/em = 660/682 nm and SYTOX Red Dead Cell Stain ex/em = 640/658. In addition, the main difference is that SYTOX Deep Red Nucleic Acid Stain has less propensity to stain RNA compared to SYTOX Red Dead Cell Stain.

For Research Use Only. Not for use in diagnostic procedures.