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  6. Invitrogen™ SYTO™ Deep Red Nucleic Acid Stain, for live cells

Invitrogen™ SYTO™ Deep Red Nucleic Acid Stain, for live cells

Catalog No. p-7180611
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Description

SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells.

  • SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells. It can be used in live-cell fluorescent imaging workflows and in fixed cell workflows, including immunocytochemistry, immunohistochemistry, and immunofluorescence experiments.
  • After a brief incubation with the stain, the nuclei of live, dead, or fixed cells fluoresce with a deep red/far red signal that is detectable with a Cy5/deep red standard filter set or laser configuration.
  • Cell-permeant dye that stains nuclei of live, dead, or fixed cells
  • Detectable with deep red/Cy5 fluorescence filter set, with excitation/emission maxima of 652/669 nm
  • Five times greater fluorescent signal when staining dsDNA vs RNA
  • SYTO Deep Red Nucleic Acid Stain can be multiplexed with blue, green, orange, red, and near-IR fluorophores when compatible fluorescent filter/laser configurations are used.
  • The stain increases in fluorescence with increasing concentrations of dsDNA. SYTO Deep Red Nucleic Acid Stain displays up to five times more fluorescence when incubated with dsDNA vs RNA, unlike DRAQ or other live-cell deep red nucleic acid stains.
  • It has a bright initial signal and excellent photo stability in a typical imaging experiment.
  • These properties make the stain ideal as a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence microscopes, fluorimeters, fluorescence microplate readers, and flow cytometers.
  • The stain has been successfully used on live monolayer cells and spheroid cells and as a nuclear counter-stain in immunocytochemistry.

Specifications

Color Deep Red
Content And Storage Store in freezer at -5°C to -30°C and protect from light.
Excitation Wavelength Range 652 nm
Dye Type Cell-Permeant
For Use With (Equipment) Fluorescence Microscope
Quantity 1 Vial
Volume (Metric) 250 μL
Detection Method Fluorescence
Emission 669 nm
Product Line SYTO
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Nucleic Acid Stain
SubCellular Localization Nucleic Acids
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Frequently Asked Questions (FAQs)

Can I use SYTO Deep Red Nucleic Acid Stain with another cell impermeant nucleic acid stain for marking live and dead cells in a population?

Yes. You may use any of the SYTOX dyes of a different fluorescent color that does not overlap with the SYTO Deep Red Nucleic Acid Stain.
Is Probenecid required to retain the SYTO Deep Red Nucleic Acid Stain within live cells?

To minimize off-target effects on other cellular functions, we recommend the use of 1X Probenecid while loading and imaging with SYTO Deep Red Nucleic Acid Stain. The ability of Probenecid to improve retention of SYTO Deep Red Nucleic Acid Stain within live cells is dependent upon the cell type; hence, the use of Probenecid is optional.

Can I incubate my live cells with the SYTO Deep Red Nucleic Acid Stain for longer periods of time?

We have not tested the SYTO Deep Red stain for cytotoxicity/mutagenicity with exposure to live cells for longer periods of time.

For Research Use Only. Not for use in diagnostic procedures.