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Thermo Scientific™ Syn-PER™ Synaptic Protein Extraction Reagent

Catalog No. PI87793
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100 mL
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PI87793 100 mL
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Catalog No. PI87793 Supplier Thermo Scientific™ Supplier No. 87793
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Description

Isolate synaptosomes containing functional synaptic proteins from neuronal tissue and primary cultured neurons with this cell lysis reagent.

Thermo Scientific™ Syn-PER Synaptic Protein Extraction Reagent is a proprietary cell lysis reagent for efficient isolation of synaptosomes containing functional synaptic proteins from neuronal tissue and primary cultured neurons.

Syn-PER Reagent is used to prepare synaptosomes containing biologically active pre-and post-synaptic proteins. When used with fresh neuronal tissue or primary cultured neurons, synaptosomes prepared with the Syn-PER Reagent can be used to study synaptic transmission. The synaptosomal proteins contained in these extracts can also be used for downstream applications such as Western blotting, immunoprecipitation, enzymatic activity assays and protein-protein interaction studies. Syn-PER Synaptic Protein Extraction Reagent minimizes the degradation of phosphoproteins and is ideal for studies requiring the preservation of phosphoprotein integrity.

Highlights:

  • Efficient extraction – obtain up to 10μg of synaptic protein per milligram of neuronal tissue or 4μg synaptic protein per 35mm dish of primary cultured neurons (106 cells)

  • Gentle formulation – isolate viable synaptosomes, extract native synaptic proteins and preserve phosphoprotein integrity

  • Fast procedure – obtain synaptosomal suspension in less than one hour

  • Simple protocol – requires no ultracentrifugation steps

Recommended for:

Isolate functional synaptosomes to study neurotransmitter release; Extract pre- and post-synaptic proteins to identify changes in protein composition and function in synapses; Preserve and study labile or transient protein phosphorylation events associated with synapses

Specifications

Quantity 100 mL
Format Liquid
Product Type Reagent
Volume (Metric) 100 mL
Content And Storage Upon receipt store product at 4°C.
Product Line Syn-PER

Frequently Asked Questions (FAQs)

Will yield be the same with cultured neurons when using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793), as compared to using fresh tissue?

We can not guarantee that the total synaptic yield from cultured neurons should be comparable to using fresh tissue.

Will yield be the same if I pellet and freeze neurons in culture when using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793), as compared to using fresh tissue?

We have tested using Syn-PER Synaptic Protein Extraction Reagent with frozen (mouse) brain tissue and total synaptic protein yields were comparable to fresh brain tissue.

Please note, frozen tissues are not recommended for synaptosome function tests such as neurotransmitter release and up take assays because synaptosomes from frozen tissues are not viable. This is a difference between frozen and fresh tissue in terms of application.

Can I perform RNA extraction after using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793)?

Yes. RNA extraction can be performed after using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793). After the lysis step, you will need to perform two centrifugations (step 4 and 5 in the manual, the first at low speed to discard the tissue or cell debris. The second one, at higher speed, will pellet synaptosomes. The supernatant arising from this step will include the cytosolic fraction (step 6).

If the synaptosomes are intact and have RNA inside, the RNA should be extractable by the kit or method of your choice.

Please note: This extraction procedure should be experimentally tested.

Can you provide the shelf-life for the Syn-PER Synaptic Protein Extraction Reagent?

The Syn-PER Synaptic Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

For Research Use Only. Not for use in diagnostic procedures.