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Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
Supplier: Thermo Scientific™ A38556
Description
- Sensitivity: low-femtogram to high-attogram
- Stability: eight hours for working solution, one year for kit at 4°C
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: up to four hours
- Recommended primary antibody concentration: 1:1000 to 1:5000 dilution (0.2 to 1.0 μg/mL)
- Recommended secondary antibody concentration: 1:100000 to 1:250000 dilution (4 to 10 ng/mL)
Specifications
| Substrate | |
| Liquid | |
| Nitrocellulose, PVDF | |
| 200 mL | |
| Low-femtogram to high-attogram | |
| Luminol/Enhancer, 100 mL, Stable Peroxide Buffer, 100 mL | |
| Wet Ice |
| Chemiluminescence | |
| Western Blot | |
| SuperSignal™ | |
| Horseradish Peroxidase | |
| 25 Mini-Gel Blots, 2000 sq. cm of Membrane | |
| SuperSignal West Atto Substrate / SuperSignal West Atto Stable Peroxide • Substrate Solution, 100 mL • Stable Peroxide Solution, 100 mL Sufficient for 25 mini-gel blots; 2000 cm2 of membrane. Store reagents at 2–8°C. |
For Research Use Only. Not for use in diagnostic procedures.