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  7. Invitrogen™ SuperScript V Reverse Transcriptase

Invitrogen™ SuperScript V Reverse Transcriptase

Catalog No. 19090050
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19090050 10,000 Units
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Catalog No. 19090050 Supplier Invitrogen™ Supplier No. 19090050

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis.

Invitrogen™ SuperScript™ V Reverse Transcriptase (RT) is a next-generation MMLV reverse transcriptase designed for fast, reliable first-strand cDNA synthesis. Building on the advanced performance of the SuperScript™ IV reverse transcriptase, SuperScript V RT combines high sensitivity, thermostability, and processivity with improved resistance to oxidation and greater workflow flexibility.

SuperScript V RT supports reliable cDNA synthesis from low-input RNA as well as highly structured, GC-rich, degraded, and inhibitor-containing samples.

Features of SuperScript V Reverse Transcriptase include:

  • Broad RNA input range—supports cDNA synthesis from 100 fg to 5 μg of RNA
  • Robust performance with challenging RNA—reaction temperatures up to 65°C help transcribe highly structured and GC-rich RNA
  • Fast cDNA synthesis—complete first-strand cDNA synthesis in as little as 1–10 minutes
  • Improved resistance to oxidation— enhanced reagent stability supports ambient shipping and room-temperature storage for up to 6 months
  • Flexible workflow—room-temperature reaction setup and stability of assembled reactions for up to 24 hours

SuperScript V RT is well suited for RT-PCR, RT-qPCR, gene expression analysis, and other applications requiring high-quality first-strand cDNA synthesis. Its enhanced reagent and assembled-reaction stability provides additional flexibility for routine, high-throughput, and automated workflows.

A DTT-free, animal origin–free formulation combined with reduced cold chain requirements further simplifies reagent handling and supports more sustainable workflows.

Specifications

Concentration 200 U/μL
Content And Storage • SuperScript V Reverse Transcriptase (50 μL)
• 5X SuperScript V Reaction Buffer (500 μL)

Store all contents at:
• -20°C for long-term storage until expiry date
• +5°C for up to 12 months
• Room temperature for up to 6 months
Detection Method qPCR, NGS, PCR
Form Liquid
Format Stand-alone Enzyme
GC-Rich PCR Performance High
PCR Method 2-step RT-qPCR, RT-PCR
Reaction Speed 10 min.
Technique Reverse Transcription
Label or Dye Not Applicable
Optimal Reaction Temperature 50°C
Product Type Reverse Transcription Reagent
Quantity 10,000 Units
Reverse Transcriptase SuperScript V
Ribonuclease H Activity Reduced
Sensitivity 0.1 pg total RNA
Shipping Condition Dry Ice, Wet Ice, Ambient
For Use With (Application) cDNA Synthesis, RT-PCR, Real Time PCR (qPCR), Sequencing, Next-Generation Sequencing (NGS)
Thermostability Up to 65°C
No. of Reactions 50 Reactions
Reaction Format Separate components
Reagent Type Reverse Transcription
Size (Final Product) Up to 20 kb
Starting Material RNA
Inhibitor Tolerance (Simple) High
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For Research Use Only. Not for use in diagnostic procedures.