missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. FERA43073

Thermo Scientific™ Super Heavy TMT Label Reagent

Catalog No. FERA43073
Change view
Click to view available options
Quantity:
2 mg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
FERA43073 2 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. FERA43073 Supplier Thermo Scientific™ Supplier No. A43073
Only null left

Description

Alternate form of TMT that contains twice the number of heavy of isotopes for labeling of peptides, and when used with TMT-labeled samples, enables 2D TMT multiplexing applications for targeted quantitation

  • The amine-reactive Super Heavy TMT Label Reagent is an alternate form of TMT that contains twice the number of heavy of isotopes for labeling of peptides, and when used with TMT-labeled samples, enables 2D TMT multiplexing applications for targeted quantitation.
  • 2-dimensional multiplexing—enables multiplexing of traditional multiplex TMT-labeled samples with super heavy-labeled peptides for multiplexed targeted quantitation
  • Improves quantitative accuracy—label reduces co-isolation and interference to enable more robust quantitation
  • Reduces acquisition complexity—heavy-labeled peptide is used as the trigger for peptide selection
  • Super Heavy TMT Label Reagent is used to label synthetic peptides and is for use specifically with TMT multiplex-labeled samples. When used in combination, the mass offset from the super heavy-labeled synthetic peptide serves as a trigger for the selection of the targeted peptide in the multiplexed sample and reduces interference/co-isolation to enable more robust quantitation.
  • This new method combines the power of multiplexing from TMT reagents with targeted quantitation methods and is referred to as the TOMAHAQ method (triggered by offset, multiplexed, accurate mass, high resolution, and absolute quantitation)1. For this application, the user is able to perform targeted quantitation on multiplexed samples for the target peptides without dependence on the chromatography conditions.
  • For high resolution analysis of the TMT-labeled peptides, the recommended LC column for a Nanospray Flex source is Acclaim PepMap 100 C18 LC Column (Cat. No. 164942 or 164939). For an EASY-Spray source, the recommended LC column is EASY-Spray C18 LC Column (Cat. No. ES802 or ES803).Reference Erickson BK, Rose CM, Braun CR, Erickson AR, Knott J, McAlister GC, Wühr M, Paulo JA, Everley RA, Gygi SP. A Strategy to Combine Sample Multiplexing with Targeted Proteomics Assays for High-Throughput Protein Signature Characterization. Mol Cell. 2017 65(2):361-370.

Specifications

Product Line TMT
Content And Storage Store in freezer (-5 to -30°C).
Label or Dye Tandem Mass Tag™ (TMT) Labels
Shipping Condition Dry Ice
Starting Material Peptides
Final Product Type Peptide
Workflow Step Protein Labeling
Quantity 2 mg

For Research Use Only. Not for use in diagnostic procedures.