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Invitrogen™ Streptavidin, Rhodamine Red™-X conjugate

Catalog No. S6366
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S6366 1 mg
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Catalog No. S6366 Supplier Invitrogen™ Supplier No. S6366
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Streptavidin, Rhodamine Red™-X conjugate

The streptavidin, Rhodamine-Red™-X conjugate has excitation and emission maxima ∼570/590 and is widely used to detect biotinylated probes by fluorescence microscopy.

Specifications

Conjugate Rhodamine Red
Quantity 1 mg
Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Shipping Condition Room Temperature
Product Line Rhodamine Red
Product Type Streptavidin Conjugate (fluorescent)
I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

For Research Use Only. Not for use in diagnostic procedures.