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Invitrogen™ Streptavidin-Horseradish Peroxidase (HRP) Conjugate

Catalog No. SA10001
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SA10001 50 μL
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Catalog No. SA10001 Supplier Invitrogen™ Supplier No. SA10001
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Description

Streptavidin-Horseradish Peroxidase (HRP) Conjugate

Streptavidin-HRP conjugate is typically used in the immunodetection of biotinylated proteins. This conjugate is suitable for use in immunoblotting, ELISA, immunomicroscopy. The amount of conjugate provided is sufficient for 25 western blots using 10 ml of working solution per reaction or 25 immunostaining reactions using 1 ml of working solution per reaction as appropriate.

Specifications

Concentration 0.75 to 1.2 mg/mL
Conjugate HRP
Quantity 50 μL
Content And Storage Store in refrigerator (2—8°C).
Form Liquid
Product Line Invitrogen
Product Type Streptavidin Conjugate (fluorescent)
Label or Dye HRP (Horseradish Peroxidase)

Frequently Asked Questions (FAQs)

Should I block my western blot membrane before incubating with Streptavidin-Horseradish Peroxidase (HRP) Conjugate (Cat. No. SA10001)?

Yes, you should block the western blot membrane before incubating with Streptavidin-HRP Conjugate. Blocking is essential to reduce non-specific binding and background signal.
We recommend incubating the membrane in 10 mL blocking solution. Gently agitate using a rocker platform for 1 hour at room temperature and then proceed to washing the membrane (Step 5 of the protocol on page 5 of the user manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/streptavidin_conjugates_man.pdf).

For Research Use Only. Not for use in diagnostic procedures.