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Invitrogen™ Streptavidin, Pacific Blue™ conjugate

Catalog No. 501121543
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Catalog No. 501121543 Supplier Invitrogen™ Supplier No. S11222
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Description

Invitrogen™'s streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ∼410/455 nm.

Invitrogen™'s streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ∼410/455 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

Specifications

Color Yellow
Conjugate Pacific Blue
Description Pacific Blue™ Conjugate Streptavidin, 1mg, Yellow, Solid Form, 407nm Absorbency, Pacific Blue™ Label or Dye, 410/455nm Excitation/Emission, Used in Blot Analysis, Flow Cytometry, Histochemical Applications, Immunoassays, –5 to –30°C Storage Temperature
Detection Method Fluorescence
Formulation Solid
pH 8
Quantity 1 mg
Regulatory Status For research use only (RUO)
Sample Volume 1 mg
Packaging 1 Each
Storage Buffer 10mg/mL PBS
Content And Storage Contains 1 vial of streptavidin Pacific Blue™ conjugate.

Store in freezer (-5 to -30°C) and protect from light.
Excitation/Emission 410/455 nm
Flow Cytometer Laser Lines 405
Shipping Condition Room Temperature
Form Solid
Product Line Pacific Blue
For Use With (Application) Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Purity or Quality Grade TLC
Product Type Streptavidin Conjugate
Label or Dye Pacific Blue
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Frequently Asked Questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.
I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

For Research Use Only. Not for use in diagnostic procedures.