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Invitrogen™ Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)

Catalog No. 501121177
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501121177 100 μL
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Catalog No. 501121177 Supplier Invitrogen™ Supplier No. S20982
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Description

Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)

The streptavidin, Alexa Fluor™ 610-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ∼496,565/627 and is widely used to detect biotinylated probes by flow cytometry. Not only are the Alexa Fluor™ 610 conjugates of R-PE are more fluorescent than the commercially available Texas Red™ conjugates of R-PE, the fluorescent emission of Alexa Fluor™ 610-R-PE tandem conjugates is shifted to somewhat longer wavelengths than is the emission of Texas Red™-R-PE conjjgates, resulting in better separation from the emission of R-PE.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.

Specifications

Concentration 1 mg/mL
Conjugate PE-Alexa Fluor 610
Detection Method Fluorescence
For Use With (Equipment) Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Quantity 100 μL
Content And Storage Contains 1 vial of streptavidin R-PE-Alexa Fluor™ 610 conjugate (1 mg/mL).

Store in refrigerator (2–8°C) and protect from light.
Excitation/Emission 488 or 561/630
Flow Cytometer Laser Lines 488 or 561
Shipping Condition Wet Ice
Form Solution
Product Line Alexa Fluor
Product Type Streptavidin Conjugate (Fluorescent)
Label or Dye PE-Alexa Fluor 610
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Frequently Asked Questions (FAQs)

Can I use my tandem dye antibody (e.g., Alexa Fluor 610-R-phycoerythrin) for microscopy?

Tandem dyes are not recommended for imaging applications. Both R-PE or APC tend to photobleach quickly and cannot be archived. Any unfolding of the phycobiliprotein would result in loss of fluorescence. They work great in flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.