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Invitrogen™ Streptavidin, Alexa Fluor™ 514 conjugate
Description
Alexa Fluor™ 514 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor™ 514 Streptavidin Conjugates:
- Alexa Fluor™ 514 streptavidin conjugate has Ex/Em maxima of ∼ (518/540)
- Bright, photostable fluorescence
- High solubility in aqueous solution
- Available in multiple colors
- Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
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Specifications
Specifications
| Conjugate | Alexa Fluor 514 |
| Quantity | 1 mg |
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Shipping Condition | Room Temperature |
| Form | Solid |
| Product Line | Alexa Fluor |
| Product Type | Streptavidin Conjugate (fluorescent) |
| Label or Dye | Alexa Fluor Dyes |
Frequently Asked Questions (FAQs)
In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.
The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).
For Research Use Only. Not for use in diagnostic procedures.