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Thermo Scientific™ Float Buoys, for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices

Catalog No. PI69588
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4 Buoys
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Catalog No. Quantity
PI69588 4 Buoys
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Catalog No. PI69588 Supplier Thermo Scientific™ Supplier No. LSG69588
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Description

Thermo Scientific Slide-A-Lyzer MINI Dialysis Device Float buoys are low density polyethylene (LPDE) rafts designed to securely hold up to 25 Slide-A-Lyzer MINI Dialysis Devices (0.1 mL only) to permit array dialysis.

Float Buoys for Slide-A-Lyzer™ MINI Dialysis Devices contain inert, low-density polyethylene (LPDE) construction that is compatible with most aqueous buffer conditions, and the floats can be washed and reused. These Float Buoys enable users to dialyze a single sample (tube) or multiple samples (25 array). Their easy-to-read column and row labels make it simple to keep track of the identity of different samples.

Float Buoys for Slide-A-Lyzer MINI Dialysis Devices are intended to hold Slide-A-Lyzer MINI Dialysis Devices (0.1 mL size only), whose bottom surfaces are a dialysis interface composed of a cellulose membrane barrier. The float holds the devices so that the bottoms contact the solution in which they are floated for dialysis. LPDE material is not suitable for use at temperatures greater than 50°C and are not designed for use as water bath floats.

Specifications

Content And Storage Store at room temperature.
Membrane Cellulose Membrane, Dialysis
Format MINI Dialysis Devices
Capacity (Metric) 0.1 mL
Product Line Slide-A-Lyzer
Quantity 4 Buoys

Frequently Asked Questions (FAQs)

With what chemicals is the Slide-A-Lyzer MINI Dialysis Unit compatible?

The Slide-A-Lyzer MINI Dialysis Unit is compatible with alcohols, ketones, esters, oxides, and solvents containing nitrogen.
Alkalis: 1N sodium hydroxide or 1N ammonium hydroxide may cause pore swelling or shrinkage; not recommended to be used with 1N potassium hydroxide
Acids: Will survive weak acids (100% acetic or formic acids), or ~25% phosphoric or sulfuric acids; it should not be used with >25% strong acids, although a weak solution of a strong acid may work
What percent of my sample should I expect to lose during dialysis when using the Slide-A-Lyzer MINI Dialysis Unit?

In general, polypropylene and regenerated cellulose have very low nonspecific binding properties. The percent recovery of a sample will depend on the unique properties of the sample and on the initial concentration of the sample.
What materials are used to manufacture the Slide-A-Lyzer MINI Dialysis Unit?

The membrane is regenerated cellulose and the plastic is a polypropylene copolymer. Both materials have very low protein and nucleic acid binding properties.

How long does it take to dialyze with the Slide-A-Lyzer MINI Dialysis Unit?

The Slide-A-Lyzer MINI Dialysis Unit can convert 100 µL of pH 2.8 buffer to pH 9.4 dialyzing against 1 liter bicarbonate buffer, pH 9.4 in less than 10 minutes.

How do I use the Slide-A-Lyzer MINI Dialysis Unit?

- Wearing gloves, apply sample (0.01-0.1 mL) using a pipette.
- OPTIONAL: Soak the Slide-A-Lyzer MINI Dialysis Unit to remove trace contaminants.
- Cap the Slide-A-Lyzer MINI Dialysis Unit (to minimize loss) and snap it into the float (sold separately).
- Insert the float into the beaker containing the dialysate.
- Recover sample.
How do I remove trace contaminants from the Slide-A-Lyzer MINI Dialysis Unit?

To remove glycerol, dialyze for 15 minutes against 1 liter of deionized water. Glycerol content is less than 3% in the 3K unit, approximately 15% in the 7K unit and 23% in the 10K unit. To remove metals, dialyze for 15 minutes against 1 liter of 1 mM EDTA. Metals present in the 3K, 7K and 10K unit include 2 ppb iron, 5 ppb magnesium, 1.5 ppb nickel, 0.2 ppb zinc, 0.2 ppb copper, 0.5 ppb chromium and 0.3 ppb cadmium. To remove sulfur, dialyze for 15 minutes against 1 liter of deionized water. Sulfur content should be minimal, less than 0.1%. Thermo Scientific quality control checks for sulfur on the membrane prior to the unit's assembly.

How do I obtain optimum performance with the Slide-A-Lyzer MINI Dialysis Unit?

Always wear gloves when touching a Slide-A-Lyzer MINI Dialysis Unit If desired, soak the Slide-A-Lyzer MINI Dialysis Unit to remove contaminants For best recovery, tilt the tube and collect the sample in the bottom corner of the device.

How can I use the Slide-A-Lyzer MINI Dialysis Unit in my laboratory?

The Slide-A-Lyzer MINI Dialysis Unit is ideal for:

- Salt reduction Buffer exchange (<10 min for pH change 2.8 to 9.5)
- Removal of small molecular weight compounds
- Micro-conjugation (reagent addition or sample evaluation anytime)
- Equilibrium dialysis (microtube, paraffin to adjust depth)
- Concentration (in a microtube concentrates @ 35-45 µl/hr; use at least a 3:1 v/v ratio of concentrator to sample.)
- Sample preparation for instrumentation analysis (HPLC, CE, MS, GC, electrophoresis, etc.)
- Sample preparation for proteins, peptides, oligonucleotides, DNA, RNA (~330 MW/base ~660 MW/bp)
- The “scavenger” technique in which a resin, membrane, polymer or protein is placed in a microtube with buffer in order to select a compound to remove from a sample in a Slide-A-Lyzer MINI Dialysis Unit.

What is the difference between your dialysis devices (plates, device, cassettes, flasks)?

Please view our selection table to choose the right dialysis device for your experiment - https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/dialysis-products.html.
What is protein dialysis?

Dialysis is the separation of small and large molecules in a solution by selective diffusion through a semi-permeable membrane. It is generally used for larger sample volumes, and can take hours to overnight for complete dialysis.