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Invitrogen™ SiteClick™ Antibody Labeling Kits DFS Item

Generate brightly labeled antibodies for flow cytometry, fluorescence imaging, or western blot detection using SiteClick™ Qdot™ R-PE, and Biotin Antibody Labeling Kits.

$632.00 - $804.00


Products 10
Catalog Number Mfr. No. Label or Dye Excitation/Emission Labeling Scale Price Quantity  
Catalog Number Mfr. No. Label or Dye Excitation/Emission Labeling Scale Price Quantity  
S10453 DFS Item
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Invitrogen™
S10453
Qdot 655 405/655 100 μg
Each for $804.00
 
S10467 DFS Item
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Invitrogen™
S10467
R-PE (R-Phycoerythrin) 496, 546, 565/578 100 μg
Each for $632.00
 
S10449 DFS Item
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Invitrogen™
S10449
Qdot 525 405/525 100 μg
Each for $770.00
 
S10454 DFS Item
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Invitrogen™
S10454
Qdot 705 405/705 100 μg
Each for $774.00
 
S10450A DFS Item
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Invitrogen™
S10450
Qdot 565 405/565 100 μg
Each for $754.00
 
S10451 DFS Item
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Invitrogen™
S10451
Qdot 585 405/585 100 μg
Each for $744.00
 
S10452 DFS Item
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Invitrogen™
LSS10452
Qdot 625 405/625 100 μg
Each for $766.00
 
S10455 DFS Item
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Invitrogen™
S10455
Qdot 800 100 μg
Each for $784.00
 
S10469A DFS Item
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Invitrogen™
S10469
Qdot 605 405/605 100 μg
Each for $716.00
 
S20033 DFS Item
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Invitrogen™
S20033
Biotin 100 to 250 μg
Each for $784.00
 
Description

Description

Generate perfectly labeled antibodies with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits. Site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies, regardless of isotype and host species. Depending upon the label, the resulting SiteClick R-PE, Qdot, or Biotin conjugated antibody can be used in flow cytometry, fluorescence imaging, or Western blot detection. Each kit contains everything needed to label 100 µg of IgG antibody with an easy-to-follow, step-by-step protocol. Achieve highly efficient, site-specific, and reproducible labeling with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits.

Conventional amine or thiol labeling chemistry can result in labeling of the antigen binding domain or compromise the structure of an antibody. SiteClick labeling technology, however, attaches the label under mild conditions to the heavy chains of an IgG antibody, ensuring that the antigen binding domains remain available for binding to your antigen target. Because SiteClick Labeling Kits allow simple and gentle site-selective attachment of detection molecules to heavy chain N-linked glycans — far from the antigen-binding domain — excellent reproducibility is achieved during sequential labelings, as well from antibody to antibody. No harsh reduction steps are required, and antibody labeling is consistent and reproducible each time it is performed.

The SiteClick R-PE, Qdot, or Biotin Antibody labeling workflow consists of three steps:

1. antibody carbohydrate domain modification,
2. azide attachment to the antibody and
3. conjugation with the DIBO-modified label.

This workflow employs copper-free click chemistry to covalently link the label containing the DIBO moiety with the azide-modified antibody. Labeling antibodies with the SiteClick antibody labeling system gives you confidence that the antibody will be labeled the same way every time, with no need for reaction optimization. This means the same number of dyes are attached to each molecule, the antigen binding site is preserved, and the same results are achieved every time.

Important features of the SiteClick Qdot Antibody Labeling Kits:

• Include everything required to label 100 µg of IgG antibody (100–250 µg of IgG antibody with the SiteClick Biotin Antibody Labeling Kit)
• Easy to follow step-by-step protocol that require only a few hours hands-on time to complete.
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugates.
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