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Applied Biosystems™ SeqStudio™ Flex Genetic Analyzer 24-Capillary Array

Catalog No. A49107
Encompass
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36 cm
50 cm
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A49107 50 cm
A49105 36 cm
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Catalog No. A49107 Supplier Applied Biosystems™ Supplier No. A49107
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An internally uncoated array for use with the SeqStudio 24 Flex Genetic Analyzer for fragment analysis, sequencing, and protein quality analysis applications. The capillaries in this array are 36 or 50 cm in length and supplied in preassembled sets of 24 capillaries per array, with a built-in frame for easy installation.

This array is an internally uncoated array for use with the SeqStudio 24 Flex Genetic Analyzer for fragment analysis, sequencing, and protein quality analysis applications. The capillaries in this array are either 36 or 50 cm in length and supplied in preassembled sets of 24 capillaries per array, with a built-in frame for easy installation.

This array is an internally uncoated array for use with the SeqStudio 24 Flex Genetic Analyzer for fragment analysis, sequencing, and protein quality analysis applications. The capillaries in this array are 50 cm in length and supplied in preassembled sets of 24 capillaries per array, with a built-in frame for easy installation.

SeqStudio Flex capillary arrays have an improved design that makes the installation process easier and more error proof compared to 3500 system capillary arrays. The improved capillary frame provides a better user experience by minimizing the risk of capillary damage and incorrect placement (figure below).

The SeqStudio Flex 50-cm arrays have been optimized for many applications and for use with POP-7, POP-6, and POP-4 polymer run modules. This provides users with the flexibility to select their ideal run times and read lengths. See the figure below for more detailed information on optimal configurations of the run module, polymer, and array length.

Like 3500 system arrays, SeqStudio Flex capillary arrays include integrated radio frequency identification (RFID) tags on the labels that enable viewing, tracking, and reporting of critical information about reagents and consumables, including usage, lot number, part number, expiration date, and on-instrument lifetime. The RFID labels also enable the Data Collection Software to remind users to replace the array when it is expired or exceeds the recommended usage limit.

SeqStudio Flex capillary arrays are specified for 160 injections.

Specifications

Capillary Length 50 cm
For Use With (Equipment) SeqStudio™ Flex 24-cap Genetic Analyzer
For Use With (Application) Sanger Sequencing and Fragment Analysis
Number of Capillaries Set of 24 capillaries
Quantity 1 array
Can I run the SeqStudio Flex Genetic Analyzer 24-Capillary Array on a SeqStudio 8 Flex Genetic Analyzer?

No, the SeqStudio Flex Genetic Analyzer 24-Capillary Array can be run only on a SeqStudio 24 Flex Genetic Analyzer.

What are the unique features of the SeqStudio Flex Series Genetic Analyzer capillary arrays?

The SeqStudio Flex Series Genetic Analyzer capillary arrays have a new array frame which provides better support for the capillaries and a tab near the array tip which makes it easier to insert into the array knob.

How often should I change the capillary array on my SeqStudio Flex Series Genetic Analyzer?

The SeqStudio Flex Series Genetic Analyzer capillary arrays are warrantied for a specific number of runs or until the expiration date printed on the array package, whichever comes first. If you notice that you are starting to get reduced data quality (e.g., shorter reads, trailing peaks in all 4 colors) even with the Sequencing Standard, these may be signs that the capillary array requires changing.

Can the capillary arrays for the SeqStudio Flex Series Genetic Analyzers be used on the 3500/3500xL Genetic Analyzer and vice versa?

No, the capillary arrays for the 3500/3500xL Genetic Analyzers and the arrays for the SeqStudio Flex Series Genetic Analyzers are not interchangeable.

How can I request assistance from Technical Support or Instrument Service for SeqStudio Flex Series Genetic Analyzers?

You can request assistance from Technical Support or Instrument Service on the SeqStudio Flex Series Genetic Analyzers via the Smart Help function. In order to use this function, access to Thermo Fisher Connect must be enabled. You can also contact Technical Support at techsupport@thermofisher.com or Service Support at instrumentsupport@thermofisher.com.

With the SeqStudio Flex Genetic Analyzer, I tried to start a run, but the instrument displays a notification that there is not enough storage space. What should I do?

The SeqStudio Flex Genetic Analyzer instrument will not run unless the required amount of space is available to save the results on the instrument. To free up space on the hard drive, you will need to delete the run histories. We recommend that you first export the results before deleting them as the process is irreversible. At the home screen, tap Actions > Run history. Select the plates that you want to delete. Tap Delete > OK to confirm.

In the SeqStudio Flex Genetic Analyzer, I do not have access to the CBC or capillary array because the CBC is currently beneath the array with the tips inserted. How can I move it so I can have access to the array or CBC?

You can move the CBC by using the Manual commands. Tap on Dashboard > Instrument Status > Manual commands. Select Move buffer container > Send command. Once the CBC is no longer beneath the array with the tips inserted, close the Manual commands screen. You will now have access to the array and CBC.

What should I do if the SeqStudio Flex Genetic Analyzer touchscreen becomes unresponsive?

If the SeqStudio Flex Genetic Analyzer touchscreen becomes unresponsive, press the power on/off button on the front of the instrument to restart the instrument. If the touchscreen is still unresponsive after the reboot, contact Technical Support at techsupport@thermofisher.com.

What should I do if the SeqStudio Flex Genetic Analyzer instrument panel is blinking amber?

If the SeqStudio Flex Genetic Analyzer instrument panel is blinking amber, you should restart the instrument.

What versions of desktop secondary analysis software can I use to analyze data generated from the SeqStudio Flex Genetic Analyzer?

The desktop secondary analysis software versions that are compatible with the SeqStudio Flex Series Genetic Analyzers are:

- Sequencing Analysis Software v7.1 or later
- SeqScape Software v4.1 or later
- Variant Reporter Software v3.1 or later
- Minor Variant Finder Software v1.2 or later
- MicrobeBridge Software v1.2 or later
- GeneMapper Software v6.1 or later

How many plates can the SeqStudio Flex Series Genetic Analyzers hold and how can I see which plate is running?

The SeqStudio Flex plate holder can hold up to 4 plates, corresponding to positions A, B, C, and D on the home screen of the software. When a plate is running, the console will display the status “Running” as well as the Time remaining on the run in the plate position. If a run has completed, the status “Completed” would be displayed with a glowing border around the plate position.

Will the SeqStudio Flex Genetic Analyzer instrument prevent a run from starting if the consumables have expired or exceeded their on-instrument shelf life?

By default, the SeqStudio Flex Genetic Analyzersare configured to allow the use of consumables beyond the expiration date and on-instrument shelf life. However, there are a couple of exceptions. The conditioning reagent is a single-use consumable. In the case of polymer, insufficient volume in the polymer pouch will prevent the run from starting. An administrator can change the configuration to prevent running if a consumable has expired or exceeded its on-instrument life. Please note that we do not recommend using consumables that exceed supported limits.

With the SeqStudio Flex Series Genetic Analyzers, can I open the drawer while a run is going on?

You can open the drawer at any time when the drawer is unlocked. When the drawer is unlocked, the screen will display “Drawer unlocked.” During sample injection, the drawer is locked, and the message displayed will be “Drawer locked.”

What is different about the SeqStudio Flex Series Genetic Analyzer plate retainers compared with the 3500 plate retainers? When shouldn't I use the 3500 plate retainers on the SeqStudio Flex Series Genetic Analyzers?

The SeqStudio Flex Series Genetic Analyzer plate retainers have one opening on the side to allow barcode reading while the 3500 plate retainers have two openings on the side which can cover the plate barcode. If the automatic barcode workflow is enabled on the SeqStudio Flex Series Genetic Analyzer, using the 3500 plate retainers will cause a barcode error in the home screen.

96-Well Standard Retainer & Base Set SeqStudio Flex Series: Cat. No. A49316
96-Well Fast Retainer & Base Set SeqStudio Flex Series: Cat. No. A49495
8-Tube Standard Retainer & Base Set SeqStudio Flex Series: Cat. No. A49296
8-Tube Fast (0.1 mL) Retainer & Base Set SeqStudio Flex Series: Cat. No. A49298
384-Well Retainer & Base Set SeqStudio Flex Series: Cat. No. A49496

Can the arrays for the 3500/3500xL Genetic Analyzers be used on the SeqStudio Flex Series Genetic Analyzers?

No, the arrays for the 3500/3500xL Genetic Analyzers cannot be used on the SeqStudio Flex Series Genetic Analyzers. The SeqStudio Flex Series Genetic Analyzercapillary array SKUs are listed below:

- Capillary array 36‑cm SeqStudio 8 Flex: Cat. No. A49104
- Capillary array 50‑cm SeqStudio 8 Flex: Cat. No. A49106
- Capillary array 36‑cm SeqStudio 24 Flex: Cat. No. A49105
- Capillary array 50‑cm SeqStudio 24 Flex: Cat. No. A49107

What consumables are shared between the SeqStudio Flex Series Genetic Analyzers and the 3500/3500xL Genetic Analyzers?

Consumables that are shared between the SeqStudio Flex Series Genetic Analyzers and the 3500/3500xL Genetic Analyzers include the Performance-Optimized-Polymer (POP), Anode Buffer Container (ABC), Cathode Buffer Container (CBC), and Conditioning Reagent.

Do I need to purchase a computer in order to run the SeqStudio Flex Series Genetic Analyzers?

There is no requirement to purchase a computer in order to run the SeqStudio Flex Series Genetic Analyzers. The Data Collection software is installed on the instrument. However, using secondary analysis software to analyze data and/or to run the Security, Audit, and Esignature (SAE) modules will require an additional computer.

What are the startup and shutdown procedures for the SeqStudio Flex Series Genetic Analyzers?

To power on the instrument, press the power on/off button on the front of the instrument. Make sure that the blue status light is on. To shut down the instrument, press the power button to turn off the instrument. If the instrument is idle for greater than 2 weeks, perform a long-term shutdown by following the Shut Down Wizard.

What are the specifications for the SeqStudio Flex Series Genetic Analyzers?

The specifications for the SeqStudio Flex Series Genetic Analyzers are:

Dimensions (W x D x H): 70 x 67.5 x 86.5 cm (uncrated)
Weight: ~115 kg
Number of Capillaries: 8 (Cat. No. A53627) or 24 (Cat. No. A53630)
Number of Dyes: 6
Sample Format: 96-well standard plates, 384-well standard plates, 96-well fast plates, 8-well standard strip tubes, and 8-well fast strip tubes

What is the SeqStudio Flex Genetic Analyzer System and what are some of its features?

The SeqStudio Flex Genetic Analyzer System is a medium-throughput fluorescence-based capillary electrophoresis system that is offered in two models. The SeqStudio 8 Flex Genetic Analyzer (Cat. No. A53627) holds the 8-capillary array and the SeqStudio 24 Flex Genetic Analyzer (Cat. No. A53630) holds the 24-capillary array. The available array lengths are 36 cm and 50 cm. The SeqStudio Flex Genetic Analyzer instruments use the Applied Biosystems SeqStudio Flex Genetic Analyzer Data Collection Software (SeqStudio Flex DCS) which resides on the instrument.

Features include:

- Onboard touchscreen computer
- 4 plate loading capacity and prioritization
- Automated barcode workflow
- Cloud connectivity capabilities enabling remote monitoring and remote plate file creation
- Voice commands
- Smart Help on-instrument support request
- Remote session capabilities