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MP Biomedicals™ SC-Ura (Powder)
Also known as Hopkins Mixture. MP Biomedicals SC-Ura (Powder) is a single dropout formulation of Synthetic Complete Supplement Mixture of amino acids for S. cerevisiae growth media. SC-Ura medium creates a richer medium for better yield and increases the
Supplier: MP Biomedicals™ 114410632
Description
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component.
SC-Ura medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. To use, add 1.9g of SC-Ura to each liter of DOB or DOBA and autoclave at 121°C for 15 minutes for complete synthetic defined medium for S.cerevisiae.
Specifications
| Powder | |
| 100 g |