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Molecular Probes™ SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750

Catalog No. S30046
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Description

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 750

The Alexa Fluor™ 750 SAIVI™ Antibody Labeling Kit provides a convenient means to label antibodies with an optimal degree of labeling for in vivo imaging applications (DOL; ∼2) over a 6-fold antibody concentration range with no adjustments in reaction volume, dye concentration, or antibody concentration necessary. Using this procedure, optimally labeled antibodies are ready for applications that require azide-free reagents, such as live-cell imaging or direct injection into animals.

Specifications

Product Type Labeling Kit
Quantity 1 Kit
Content And Storage Store in refrigerator 2°C to 8°C and protect from light.
Detection Method Fluorescence
Excitation/Emission 749/775
Labeling Scale 0.5 to 3 mg
Shipping Condition Room Temperature
Product Line Alexa Fluor, SAIVI
Label or Dye Alexa Fluor 750
Color Infrared
Labeling Target Antibodies
Label Type Alexa Fluor Dyes
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Frequently Asked Questions (FAQs)

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.
What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.
What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

For Research Use Only. Not for use in diagnostic procedures.