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Molecular Probes™ SAIVI™ Alexa Fluor™ 647 Antibody/Protein 1 mg-Labeling Kit

Catalog No. S30044
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Description

The SAIVI Alexa Fluor 647 Antibody/Protein Labeling Kit provides the materials needed conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye for in vivo animal imaging applications.

The SAIVI Alexa Fluor™ 647 Antibody/Protein 1 mg-Labeling Kit provides a convenient means to label proteins with Alexa Fluor™ 647 near-IR emitting dye. The kit is optimized for labeling and purifying 1 mg of IgG per conjugation reaction; comparable amounts of other proteins (>30 kDa) can also be labeled. To conveniently control the degree of labeling (DOL), this kit includes a DOL modulating reagent and instructions for decreasing the DOL from its intrinsic highest value. Using this method, protein preparations with varying ratios of dye to protein can be quickly and reproducibly obtained, allowing more efficient optimization in applications such as in vivo imaging, where the DOL of a protein can have significant effects on the signal-to-background ratio, biodistribution, and clearance.

Specifications

Product Type Labeling Kit
Quantity 1 kit
Content And Storage Store in refrigerator 2°C to 8°C and protect from light.
Detection Method Fluorescence
Labeling Scale 1 mg
Shipping Condition Room Temperature
Product Line Alexa Fluor, SAIVI
Label or Dye Alexa Fluor 647
Labeling Target Antibodies, Proteins

Frequently Asked Questions (FAQs)

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.
What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.
What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

For Research Use Only. Not for use in diagnostic procedures.