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Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Specifications

For Use With (Application) For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
Storage Requirements 2°C to 8°C
Product Type rProtein A Sepharose Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
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Products 3
Catalog Number Mfr. No. Quantity Binding Capacity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Binding Capacity Price Quantity & Availability  
45002972
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Cytiva
17127902
25 mL 50 mg Human lgG/mL N/A
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45002971
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Cytiva
17127901
5 mL 50 mg Human lgG/mL medium2 N/A
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45002973
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Cytiva
17127903
200 mL 50 mg Human lgG/mL medium2 N/A
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Description

Description

  • Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
  • High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
  • No mammalian components involved during manufacturing process
  • Matrix: 4% cross-linked agarose
  • Average particle size: 90μm (d50V)
  • Ligand: 6mg rProtein A/mL medium (E. coli)
  • Binding capacity: ∽50mg human IgG/mL media
  • pH stability working range: 3 to 10
  • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed
Specifications

Specifications

For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
2°C to 8°C
150 to 250 cm/Hoμr
9.84 in.
100 kPa
2 to 9
Recombinant Protein A (E. coli)
Stable in aqueous Buffers commonly used in Protein A Chromatography
rProtein A Sepharose Fast Flow Media
60 to 165 μm
25 cm
4% Cross-Linked Agarose
20% Ethanol
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