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  5. Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Catalog No. 45002972
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Quantity:
5 mL
25 mL
200 mL
Binding Capacity:
50 mg Human lgG/mL
50 mg Human lgG/mL medium2
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Catalog No. Quantity Binding Capacity
45002972 25 mL 50 mg Human lgG/mL
45002971 5 mL 50 mg Human lgG/mL medium2
45002973 200 mL 50 mg Human lgG/mL medium2
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Catalog No. 45002972 Supplier Cytiva Supplier No. 17127902
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Description

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

  • Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
  • High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
  • No mammalian components involved during manufacturing process
  • Matrix: 4% cross-linked agarose
  • Average particle size: 90μm (d50V)
  • Ligand: 6mg rProtein A/mL medium (E. coli)
  • Binding capacity: ∽50mg human IgG/mL media
  • pH stability working range: 3 to 10
  • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed

Specifications

Quantity 25 mL
For Use With (Application) For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
Storage Requirements 2°C to 8°C
Binding Capacity 50 mg Human lgG/mL
Product Type rProtein A Sepharose Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
Particle Size 60 to 165 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix 4% Cross-Linked Agarose
pH Range 2 to 9
Storage Buffer 20% Ethanol
Ligand Type Recombinant Protein A (E. coli)
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