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Invitrogen™ RNAqueous™ Total RNA Isolation Kit
The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10^2–10^7 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
Supplier: Invitrogen™ AM1912
Description
The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
The RNAqueous kit provides:
- Convenient syringe-based filtration
- Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
Identifiants chimiques
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Room Temperature |
Spécifications
| • 6 mL Formaldehyde Load Dye; store at -20°C • 60 mL Lysis/Binding Solution; 4°C • 60 mL Water; 4°C • 4 mL Lithium Chloride Precipitation Solution; 4°C • 40 mL Wash Solution #1; 4°C • 80 mL Wash Solution #2/3 (concentrated); 4°C • 50 Filter Cartridges; room temperature • 100 Collection Tubes; room temperature • 10 mL Elution Solution; room temperature |
|
| 50–120 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| 50 Preps | |
| Bacteria: ≤109 cells, Cells: ≤107, Tissue: ≤75 mg, Plant: ≤75 mg, Yeast: ≤108 cells, Virus: varies, Enzymatic Reactions: varies |
| Spin Column (Glass Fiber Filter) | |
| Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions | |
| Not High-throughput Compatible (Manual) | |
| RNA Isolation Kit | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.