Learn More
Invitrogen™ RiboMinus™ Bacteria 2.0 Transcriptome Isolation Kit
Description
Highly efficient depletion of 16S, 23S, and 5S rRNA from a variety of species
The RiboMinus Bacteria 2.0 Transcriptome Isolation Kit is a complete and automatable kit that enables efficient and selective removal of bacterial and archaebacterial ribosomal RNA (rRNA) from intact total RNA (100 ng to 5 μg) in less than one hour.
Key features of the RiboMinus Bacteria 2.0 kit:
- Efficient—the included RiboMinus Pan-Prokaryote Probe Mix has consistently shown less than 10% of reads mapping to 16S and 23S rRNA reads
- Wide range of targets—designed with >150 probe designs targeting 16S, 23S, and 5S rRNA targets from >76 representative species of bacteria and archaebacteria from common phyla and sub-phyla
- Convenient—Dynabeads MyOne Streptavidin C1 magnetic beads and bead cleanup module and protocols included for Kingfisher instruments
- Flexible— accommodates mass inputs from 100 ng to 5 μg of total RNA; works with wide range of total RNA samples with RIN values from 5.0–10.0
- High detection sensitivity—increases the number of sequencing reads mapping to protein-coding transcripts compared to previous version
- Suitable for metatranscriptomics—deplete rRNA of many microbial species in metagenomic samples
Target rRNA from panorama of bacteria and archaebacteria
The RiboMinus Bacteria 2.0 kit is based on hybridization-capture technology and includes a new RiboMinus Pan-Prokaryote Probe Mix, which results in a 4-fold decrease of ribosomal reads remaining and shows significant improvement over the previous bacterial probe mix. The new probe mix consists of over 150 3’-biotin-labeled ssDNA oligonucleotiode designs. These designs were created using highly conserved regions of rRNA across 76 pan-prokaryote species (see table below) and tested with several species (see compatibility list below).
Dynabeads MyOne Streptavidin C1 cleanup kit included
Once the probe mix has selectively hybridized to the target rRNA transcripts, removal of the probe-rRNA complex is completed by capture on Dynabeads MyOne Streptavidin C1 magnetic beads, leaving behind rRNA-depleted RNA. The RiboMinus Bacteria 2.0 kit includes a magnetic bead-based cleanup module for concentration of the rRNA-depleted RNA, enabling easier handling, scalability, and automation on KingFisher instruments. The resulting rRNA-depleted RNA (RiboMinus RNA) is suitable for whole-transcriptome analysis by RNA-Seq or other downstream applications.
Specifications
Specifications
| Content And Storage | RiboMinus Isolation Kits include: RiboMinus Magnetic Beads, RiboMinus human/mouse, bacteria, or yeast probe, and Hybridization Buffer The optional RiboMinus Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at 4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored. |
| Isolation Technology | Magnetic Bead |
| Sample Type | Total RNA (Bacterial) |
| Final Product Type | Transcriptome RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Next Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction |
| No. of Reactions | 12 reactions |
| Product Line | RiboMinus |
| Quantity | 12 reactions |
| Starting Material Amount | 100 ng to 5 ug of total RNA |
| Show More |
Frequently Asked Questions (FAQs)
The RiboMinus Pan-Prokaryote Probe Mix is a DNA probe and is shipped on dry ice (while the rest of the kit is shipped at 4 degrees C). The RiboMinus Pan-Prokaryote Probe Mix is stable for at least a year when stored at -20 degrees C.
For species compatibility inquiries (not already listed on the product page), please send an email to techsupport@thermofisher.com and provide a copy of your species rRNA FASTA sequences.
We typically recover approximately 3-6% of input total RNA following the rRNA depletion, concentration, and purification steps.
Use 1 µL (100 pmoles) RiboMinus Pan-Prokaryote Probe Mix with 100 ng to < 1 µg total RNA sample input. Use 3 µL (300 pmoles) RiboMinus Pan-Prokaryote Probe Mix with 1 µg to 5 µg total RNA sample input.
We recommend isolating total RNA using one of the following methods: - TRIzol Plus RNA Purification Kit (Cat. No. 12183555) - PureLink RNA Mini Kit (Cat. No. 12183018A, 12183020, 12183025) - MagMAX mirVana Total RNA Isolation Kit (Cat. No. A27828).
For research use only. Not for use in diagnostic procedures.