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Invitrogen™ Rhodopsin Recombinant Rabbit Monoclonal Antibody (Rho 1D4)
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Catalog No. PIMA547925
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200 μg
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Catalog No. Quantity
PIMA547925 200 μg
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Catalog No. PIMA547925 Supplier Invitrogen™ Supplier No. MA547925
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Description

Rabbit Recombinant Monoclonal Antibody

Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

Specifications

Antigen Rhodopsin
Applications ELISA, Immunohistochemistry, Immunoprecipitation, Radioimmune Assays (RIA), Western Blot, Immunocytochemistry
Classification Recombinant Monoclonal
Clone Rho 1D4
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.02% ProClin 300
Gene RHO
Gene Accession No. P02699, P08100, P15409, P35359, P51489
Gene Alias CSNBAD1; L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; Noerg1; OPN 2; Opn2; Ops; opsin 2; opsin 2, rod pigment; opsin-2; Red Opsin; retinal rod opsin pigment rh1.1; Rh; RH1; rh1.1; rho; rho protein; rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); rhodopsin-like-1.1 rod opsin; Rod Opsin; RP 4; RP4; seven transmembrane light receptor; unknown opsin-like protein; wu:fi06d11; zfo2; zfrho
Gene Symbols RHO
Host Species Rabbit
Immunogen Bleached bovine rod outer segment (ROS).
Purification Method Protein A
Quantity 200 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 212541, 24717, 30295, 509933, 6010
Target Species Human, Mouse, Rat, Bovine, Amphibian, Zebrafish
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG κ
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