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  6. Invitrogen™ RELM alpha Monoclonal Antibody (DS8RELM), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Invitrogen™ RELM alpha Monoclonal Antibody (DS8RELM), Alexa Fluor™ 700, eBioscience™, Invitrogen™
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Catalog No. 56544182
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100 μg
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56544182 100 μg
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Catalog No. 56544182 Supplier Invitrogen™ Supplier No. 56544182

Description

Rat Monoclonal Antibody

Description: This DS8RELM monoclonal antibody recognizes mouse RELM alpha, also known as FIZZ1. This antibody was shown not to cross-react with mouse RELM gamma. Applications Reported: This DS8RELM antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This DS8RELM antibody has been tested by intracellular staining followed by flow cytometric analysis of resident peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins located at www.thermofisher.com/flowprotocols. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

Specifications

Antigen RELM alpha
Applications Flow Cytometry
Classification Monoclonal
Clone DS8RELM
Concentration 0.2 mg/mL
Conjugate Alexa Fluor 700
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Retnla
Gene Accession No. Q9EP95
Gene Alias 1810019L16Rik; cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; Fizz1; Fizz-1; FIZZ2; found in inflammatory zone 1; Himf; HXCP2; Hypoxia-induced mitogenic factor; parasite-induced macrophage novel gene 1 protein; Pmng1; RELMa; RELMalpha; RELM-alpha; RELMb; RELMbeta; RELM-beta; resistin like alpha; resistin-like alpha; Retnla; Xcp2
Gene Symbols Retnla
Host Species Rat
Immunogen Recombinant mouse RELM alpha protein.
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 57262
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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