Invitrogen™ ReadyProbes™ Reagent F-Actin Phalloidin Conjugates

Description

ActinGreen 488 ReadyProbes Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor 488 dye. ActinGreen 488 reagent is a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) packaged in a dropper bottle. ActinRed 555 ReadyProbes Reagent is a comparable F-actin stain conjugated to the red-orange fluorescent dye tetramethylrhodamine (TRITC). ActinRed 555 reagent is a room temperature-stable solution of Rhodamine Phalloidin (Cat. No. R415) packaged in a dropper bottle.

These ready-to-use solutions combines superior brightness with exceptional F-actin staining. Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals and low non-specific binding.

Features

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• ActinGreen 488 Excitation/Emission: (495/518 nm), detected through standard GFP and FITC filter sets
• ActinRed 555 Excitation/Emission: (540/565 nm), detected through standard RFP and TRITC filters sets
• Use counterstain (i.e., DAPI or NucBlue ReadyProbes reagent) as desired

ReadyProbes format advantages

• Ready-to-use formulation—no need to dilute, weigh, or pipette
• Just use two drops per mL of medium to stain, incubate 30 minutes, wash, then image
• Stable at room temperature—keep handy on the benchtop

Applications

• For fixed and permeabilized cells or tissue
• Fluorescence microscopy (IF/IHC/ICC)
• High content screening, high content imaging
• Flow cytometry

Suggestions for use

• Both ActinRed 555 and ActinGreen 488 reagents may be added directly to fixed cells in full medium or buffer solutions
• In most cases 2 drops/mL and an incubation time of 15–30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.